IND Antibody

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Code	CSB-PA525724XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) IND Polyclonal antibody

Uniprot No.

O81313

Target Names

IND

Alternative Names

IND antibody; BHLH40 antibody; EN120 antibody; At4g00120 antibody; F6N15.18Transcription factor IND antibody; Basic helix-loop-helix protein 40 antibody; AtbHLH40 antibody; bHLH 40 antibody; Protein INDEHISCENT antibody; Transcription factor EN 120 antibody; bHLH transcription factor bHLH040 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana IND protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Transcription regulator required for seed dispersal. Involved in the differentiation of all three cell types required for fruit dehiscence. Acts as the key regulator in a network including SHP and ALC that controls specification of the valve margin. Works with ALC, SHP, and FUL to allow differentiation of the lignified valve layer, the spring-loaded mechanism of fruit that promotes opening. Regulates the expression of the YJ80 marker.

Gene References into Functions

ALCATRAZ is a negative regulator of INDEHISCENT, thereby shedding new light on how ALCATRAZ drives separation layer formation. PMID: 24004048
INDEHISCENT is part of a regulatory network that specifies valve margin /dehiscence zone identity in Arabidopsis thaliana. PMID: 23126654
SPT and IND at least partially mediate their joint functions in gynoecium and fruit development by controlling auxin distribution. PMID: 21990939
Study show that IND may directly regulate SPT expression through variant E-box. PMID: 20176890
results demonstrate a cellular mechanism for establishing the distinct cell types and a role for IND in regulating the unequal cell divisions in the seven-layer zone PMID: 16845525
INDEHISCENT directly binds the promoter of PINOID kinase to regulate auxin transport via the PINFORMED auxin efflux transporters. In this way IND generates an auxin minima at the valve margins, which is required for valve margin development. PMID: 19478783

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Subcellular Location

Nucleus.

Tissue Specificity

After fertilization, it is expressed in stripes about four cells wide at the margins of developing wild-type fruit. Also expressed in the inner valve layer, which becomes lignified later in fruit development. Detected in roots.

Database Links

KEGG: ath:AT4G00120

STRING: 3702.AT4G00120.1

UniGene: At.34598

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IND Antibody

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