Recombinant Arabidopsis thaliana Transcription factor IND (IND)

Code CSB-YP525724DOA
MSDS
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Source Yeast
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Code CSB-EP525724DOA
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Source E.coli
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Code CSB-EP525724DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525724DOA
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Source Baculovirus
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Code CSB-MP525724DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IND
Uniprot No.
Alternative Names
IND; BHLH40; EN120; At4g00120; F6N15.18Transcription factor IND; Basic helix-loop-helix protein 40; AtbHLH40; bHLH 40; Protein INDEHISCENT; Transcription factor EN 120; bHLH transcription factor bHLH040
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-198
Target Protein Sequence
MENGMYKKKG VCDSCVSSKS RSNHSPKRSM MEPQPHHLLM DWNKANDLLT QEHAAFLNDP HHLMLDPPPE TLIHLDEDEE YDEDMDAMKE MQYMIAVMQP VDIDPATVPK PNRRNVRISD DPQTVVARRR RERISEKIRI LKRIVPGGAK MDTASMLDEA IRYTKFLKRQ VRILQPHSQI GAPMANPSYL CYYHNSQP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription regulator required for seed dispersal. Involved in the differentiation of all three cell types required for fruit dehiscence. Acts as the key regulator in a network including SHP and ALC that controls specification of the valve margin. Works with ALC, SHP, and FUL to allow differentiation of the lignified valve layer, the spring-loaded mechanism of fruit that promotes opening. Regulates the expression of the YJ80 marker.
Gene References into Functions
  1. ALCATRAZ is a negative regulator of INDEHISCENT, thereby shedding new light on how ALCATRAZ drives separation layer formation. PMID: 24004048
  2. INDEHISCENT is part of a regulatory network that specifies valve margin /dehiscence zone identity in Arabidopsis thaliana. PMID: 23126654
  3. SPT and IND at least partially mediate their joint functions in gynoecium and fruit development by controlling auxin distribution. PMID: 21990939
  4. Study show that IND may directly regulate SPT expression through variant E-box. PMID: 20176890
  5. results demonstrate a cellular mechanism for establishing the distinct cell types and a role for IND in regulating the unequal cell divisions in the seven-layer zone PMID: 16845525
  6. INDEHISCENT directly binds the promoter of PINOID kinase to regulate auxin transport via the PINFORMED auxin efflux transporters. In this way IND generates an auxin minima at the valve margins, which is required for valve margin development. PMID: 19478783

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Subcellular Location
Nucleus.
Tissue Specificity
After fertilization, it is expressed in stripes about four cells wide at the margins of developing wild-type fruit. Also expressed in the inner valve layer, which becomes lignified later in fruit development. Detected in roots.
Database Links

KEGG: ath:AT4G00120

STRING: 3702.AT4G00120.1

UniGene: At.34598

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