Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Indy Polyclonal antibody

Uniprot No.

Q9VVT2

Target Names

Indy

Alternative Names

Indy antibody; CG3979Protein I'm not dead yet antibody; INDY transporter protein antibody; drIndy antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Indy protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na(+), K(+), or Cl(-). Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.

Gene References into Functions

Our data suggest INDY may be a physiological regulator that modulates intermediary metabolism in response to changes in nutrient availability and organismal needs by modulating dPGC-1. PMID: 24827528
Evolution reaffirms that the mechanism of heterozygote advantage has acted upon the Indy gene to assure increased reproductive fitness and, coincidentally, longer life span through regulatory transposon mutagenesis. PMID: 24519859
drIndy is a cation-independent electroneutral transporter for a variety of tricarboxylic acid-cycle intermediates, with preference for citrate compared with succinate. PMID: 12186628
INDY functions as a novel sodium-independent mechanism for transporting Krebs and citric acid cycle intermediates through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage PMID: 12391301
Indy mutation causes a decrease in the slope of the mortality curve consistent with a slowing in the rate of aging without a concomitant reduction in resting metabolic rate, flight velocity, or age-specific fecundity. PMID: 12626742
the transporter encoded by the life-extending gene Indy is a disulphonic stilbene-sensitive, sodium-independent anion exchanger that is capable of exchanging pairs of dicarboxylates and citrate across the plasma membrane PMID: 16608441
Does not influence lifespan in Drosophila after correction for genetic and cytoplasmic background effects. PMID: 17571923
Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage PMID: 19164521
Study concludes that Indy and calorie restriction (CR) interact to affect longevity and that a decrease in Indy may induce a CR-like status that confers life span extension. PMID: 19470468

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Subcellular Location

Basolateral cell membrane; Multi-pass membrane protein. Note=Basolateral membrane of cells in the midgut.

Protein Families

SLC13A/DASS transporter (TC 2.A.47) family, NADC subfamily

Tissue Specificity

In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the t

Database Links

KEGG: dme:Dmel_CG3979

STRING: 7227.FBpp0074848

UniGene: Dm.2528

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Indy Antibody

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