Recombinant Drosophila melanogaster Protein I'm not dead yet (Indy), partial

Code CSB-YP896156DLU
MSDS
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Source Yeast
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Code CSB-EP896156DLU
MSDS
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Source E.coli
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Code CSB-EP896156DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896156DLU
MSDS
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Source Baculovirus
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Code CSB-MP896156DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Indy
Uniprot No.
Alternative Names
Indy; CG3979Protein I'm not dead yet; INDY transporter protein; drIndy
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na(+), K(+), or Cl(-). Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.
Gene References into Functions
  1. Our data suggest INDY may be a physiological regulator that modulates intermediary metabolism in response to changes in nutrient availability and organismal needs by modulating dPGC-1. PMID: 24827528
  2. Evolution reaffirms that the mechanism of heterozygote advantage has acted upon the Indy gene to assure increased reproductive fitness and, coincidentally, longer life span through regulatory transposon mutagenesis. PMID: 24519859
  3. drIndy is a cation-independent electroneutral transporter for a variety of tricarboxylic acid-cycle intermediates, with preference for citrate compared with succinate. PMID: 12186628
  4. INDY functions as a novel sodium-independent mechanism for transporting Krebs and citric acid cycle intermediates through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage PMID: 12391301
  5. Indy mutation causes a decrease in the slope of the mortality curve consistent with a slowing in the rate of aging without a concomitant reduction in resting metabolic rate, flight velocity, or age-specific fecundity. PMID: 12626742
  6. the transporter encoded by the life-extending gene Indy is a disulphonic stilbene-sensitive, sodium-independent anion exchanger that is capable of exchanging pairs of dicarboxylates and citrate across the plasma membrane PMID: 16608441
  7. Does not influence lifespan in Drosophila after correction for genetic and cytoplasmic background effects. PMID: 17571923
  8. Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage PMID: 19164521
  9. Study concludes that Indy and calorie restriction (CR) interact to affect longevity and that a decrease in Indy may induce a CR-like status that confers life span extension. PMID: 19470468

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Subcellular Location
Basolateral cell membrane; Multi-pass membrane protein. Note=Basolateral membrane of cells in the midgut.
Protein Families
SLC13A/DASS transporter (TC 2.A.47) family, NADC subfamily
Tissue Specificity
In adults, abundantly expressed in the fat body, basolateral region of midgut cells and oenocytes. Low level expression is seen in the halteres, procardia, restricted regions of the esophagus and hindgut, base of the legs and in a subset of cells in the t
Database Links

KEGG: dme:Dmel_CG3979

STRING: 7227.FBpp0074848

UniGene: Dm.2528

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