daf-2 Antibody

Code CSB-PA853367XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans daf-2 Polyclonal antibody
Uniprot No.
Target Names
daf-2
Alternative Names
daf-2 antibody; Y55D5A.5 antibody; Insulin-like receptor antibody; IR antibody; EC 2.7.10.1 antibody; Abnormal dauer formation protein 2) [Cleaved into: Insulin-like receptor subunit alpha; Insulin-like receptor subunit beta] antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans daf-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Insulin receptor-like tyrosine kinase which regulates metabolism, controls longevity and prevents developmental arrest at the dauer stage. Binding of INS family members may either stimulate, or antagonize, association of the receptor with downstream mediators such as pdk-1 and age-1. Required for germline progenitor proliferation during larval development. Required for the response to environmental stimuli such as light, food, pheromone, and temperature. Negatively regulates resistance to UV and oxidative stress. In a daf-16/FOXO-dependent manner, plays a role in regulating the response to white light. Role in immune function and pathogen resistance. Negatively regulates autophagy. Regulates daf-18/PTEN protein levels. Plays a role in controlling seam cell development during the larval stages.
Gene References into Functions
  1. neuron-class-specific alternative splicing determined by the combinatorial action of RBFOX proteins (ASD-1 and FOX-1), UNC-75/CELF and PTB-1/PTB PMID: 27198602
  2. Suggest mbk-1 as a novel regulator of C. elegans longevity upon both, germline ablation and DAF-2 inhibition, and provide evidence for mbk-1 regulating DAF-16 activity in germline-deficient animals. PMID: 28562327
  3. Five MMP alleles proved to be novel dauer-enhancing mutations, including one new allele in the previously uncharacterized C-terminus of DAF-2 CRISPR-Cas9 genome editing was used to confirm the phenotypic consequence of six of these DAF-2 mutations and to replicate an allelic series of known human disease mutations in a highly conserved tyrosine kinase active site residue PMID: 27856697
  4. Cholesterol is required for the fasting-induced upregulation of transcriptional target genes such as the insulin/IGF-1 pathway effector DAF-16, and cholesterol deprivation suppresses the long lifespan of the insulin/IGF-1 receptor daf-2 mutant. Remarkably, we found that cholesterol plays an important role in the fasting-induced nuclear accumulation of DAF-16. PMID: 27989925
  5. this study shows that heat shock increases the life span of wild type Caenorhabditis elegans upon pathogenic encounter; similar increase in resistance towards pathogenesis observed in long-lived C. elegans daf-2 mutants PMID: 27317398
  6. this study shows that daf-2(-) mutants are hyper-immune and exhibit reduced pathological consequences during challenge with Coxiella burnetii PMID: 27884946
  7. Results indicates a contribution of daf-2-mediated insulin signalling in the transition from local area search to dispersal in food-associated behaviour in adult C. elegans PMID: 27209024
  8. The long-lived mutant daf-2(m41) worms are metabolically youthful compared to wild-type worms, but this relative youth only appears in middle age. PMID: 26381038
  9. daf-2(e1370) mutation improves quality of life and lifespan extension. PMID: 26586186
  10. These results reveal a new function for daf-2 signaling, and, most importantly, demonstrate that genetic programming contributes to freezing survival. PMID: 26635120
  11. Insulin signalling regulates gene expression during cold tolerance. Insulin released from pheromone-sensing neurons is received by the intestine and neurons regulating gene expression for cold habituation. PMID: 25048458
  12. When daf-2 animals were incubated at 20 degrees C they remained long-lived, but nuclear appearance changed at similar rate as in the wild type. PMID: 24671263
  13. Integrated our proteomic data set together with a selection of other data in an attempt to reconstruct and resolve the intricacies of altered daf-2 metabolism. PMID: 24555535
  14. in the presence of food, the DAF-2 insulin-like receptor signals through the RAS-ERK pathway to drive meiotic prophase I progression and oogenesis; in the absence of food, there is downregulation of the RAS-ERK pathway, and oogenesis is stalled. PMID: 24120884
  15. Reconciliation of daf-2 suppression by elt-3 in Caenorhabditis elegans from Tonsaker et al. (2012) and Kim et al. (2012). PMID: 23262285
  16. The modulation of DAF-2 protein level by nutritional status may constitute an important component in the irreversible commitment to dauer arrest. PMID: 22123849
  17. The daf-2 insulin-signaling mutant is able to reduce uterine masses at extreme age. PMID: 22562940
  18. Fluorodeoxyuridine affects the identification of metabolic responses to daf-2 status in Caenorhabditis elegans. PMID: 22116032
  19. The daf-2 pathway suppressed vitellogenesis through transcriptional mechanisms reflecting reproductive phenotypes, as well as distinct posttranscriptional mechanisms. PMID: 21749693
  20. Data show that glucose stress, oga-1 mutants, and ogt-1 mutants each result in an increase in daf-2 carbohydrate storage. PMID: 21441213
  21. Proteomic analyses of the environmentally resistant and 'nonaging' C. elegans dauer stage and long-lived daf-2 mutants has provided a unique insight into the protein changes which mediate survival against endogenously produced toxins. PMID: 20392130
  22. daf-2(e1370) and daf-2(e1370);pept-1(lg601) animals were compared with a focus on genes that showed significantly higher changes in expression levels in daf-2;pept-1 than in daf-2. PMID: 20550516
  23. These results suggest that polyunsaturated fatty acids (PUFA) act as mediators of daf-2/insulin signaling and that daf-16 might be involved in fatty acid homeostasis under the control of PUFA. PMID: 20226839
  24. Here we show that the C. elegans Insulin/IGF-1 receptor mutant daf-2 improves memory performance early in adulthood and maintains learning ability better with age but, surprisingly, demonstrates no extension in long-term memory with age. PMID: 20502519
  25. analysis of how divergent mechanisms control hypoxic sensitivity and lifespan by the DAF-2/insulin/IGF-receptor pathway PMID: 19936206
  26. daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the signaling pathway of this protein PMID: 12654727
  27. daf-2 mutants were resistant to killing by Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa PMID: 12817143
  28. Role of DAF-2 in longevity and aging, and interactions with DAF-16, are reviewed. PMID: 14516929
  29. findings show that reducing daf-2 mutant activity further by subjecting weak daf-2 mutants as young larvae to dal-2 RNA interference (RNAi) produced larger increases in life-span without triggering dauer formation PMID: 14576426
  30. The DAF-2 pathway happens in several organisms and mounted drug discovery programs around those targets. PMID: 15217602
  31. DAF-2 signaling controls mitochondrial bioenergetics by adjusting the rate of ATP synthesis to the rate of ATP utilization and by regulating the heat-producing proton leak pathway. PMID: 15708444
  32. Data show that an activated Ras mutation, let-60, weakly suppresses constitutive dauer diapause in daf-2 and age-1 (PI3K) mutants. PMID: 16164423
  33. REVIEW: role of DAF-2/insulin signaling on lifespan PMID: 16441841
  34. daf-2 has a role in conversion of fat to carbohydrate, and conservation of ATP stocks PMID: 16522328
  35. isolated dauer-formation mutations in C. briggsae, a species closely related to the model organism C. elegans and identified mutations in orthologs of C. elegans genes daf-2, daf-3 and daf-4 PMID: 17660533
  36. 86 proteins identified that were more or less abundant in long-lived daf-2 mutant worms; genetic studies on a subset of the proteins indicated they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage & aging PMID: 17673661
  37. Gene silencing dramatically shortens daf-2 mutant longevity but less dramatically shorten daf-2 life span. PMID: 18006689
  38. mutation of daf-12 marginally reduced life span in daf-2(+) hermaphrodites, as in plate-cultured populations, it did not in daf-2(+) males. PMID: 18056973
  39. consolidate and extend our understanding of the complex genetics of daf-2 and its underlying molecular biology in Caenorhabditis elegans. PMID: 18245374
  40. Hypoxia-mediated defects in nervous system development depended on signaling through the insulin-like receptor DAF-2, which serves to control the level of reactive oxygen species that also affects axon pathfinding. PMID: 18587389
  41. Pathogen resistance of daf-2, akt-1, and akt-2 mutants is associated with restricted bacterial colonization, and daf-2 mutants are better able to clear an infection after challenge with Pseudomonas aeruginosa. PMID: 18782349
  42. Pseudomonas aeruginosa suppresses host immunity by activating the DAF-2 insulin-like signaling pathway in Caenorhabditis elegans. PMID: 18927620

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database Links

KEGG: cel:CELE_Y55D5A.5

STRING: 6239.Y55D5A.5a

UniGene: Cel.8775

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