Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Inx2 Polyclonal antibody

Uniprot No.

Q9V427

Target Names

Inx2

Alternative Names

Inx2; prp33; CG4590; Innexin inx2; Innexin-2; Gap junction protein prp33; Pas-related protein 33

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Inx2 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Structural components of the gap junctions. Involved in gap junctional communication between germline and somatic cells which is essential for normal oogenesis. In embryonic epidermis, required for epithelial morphogenesis. Required for keyhole formation during early stages of proventriculus development in response to wg signaling. In follicle cells, promotes the formation of egg chambers in part through regulation of shg and baz at the boundary between germ cells and follicle cells. In inner germarial sheath cells, required for survival of early germ cells and for cyst formation.

Gene References into Functions

Inx2 mediated calcium flux in the follicle cells stimulates endocytosis by altering Dynamin (Shibire) distribution which is critical for careful calibration of STAT activation and, thus for border cell specification. PMID: 28114410
inx2 is required for the transcriptional activation of dpp and punt in the eye disc. PMID: 26455410
Downregulation of either ogre or inx2 selectively in glia, by targeted expression of RNA interference transgenes, leads to a significant reduction in the size of the larval nervous system and behavioural defects in surviving adults. PMID: 23813964
Data propose that Inx3 is a critical factor for dorsal closure and that it mediates the stability of Inx1, 2 and DE-cadherin by forming a complex. PMID: 23894431
Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila. PMID: 22001874
role of the innexin 2 and ogre genes during gastrointestinal development of the fly embryo PMID: 12064608
first study of cellular distribution and protein-protein interactions of an innexin gap junctional channel protein (inx2) in the developing epithelia of Drosophila PMID: 15047872
innexin 2 is positioned apico-laterally as well as apically between follicle cells and germ-line cells. Innexin 2 is participating in cell-cell communication via hemichannels residing in the oolemma PMID: 19038051

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Cytoplasm. Apical cell membrane. Apicolateral cell membrane. Basolateral cell membrane. Lateral cell membrane. Note=In ovary, localizes to the apicolateral membrane between follicle cells and to the apical membrane between follicle cells and germline cells. Accumulates in the apicolateral membrane of epithelial cells in the epidermis. In salivary gland cells, accumulates in the basolateral membrane. In hindgut epithelial cells, accumulates in the lateral membrane. In nurse cells, localizes around the nuclei at oogenesis stage 10a and then, during stages 10b and 11, in cytoplasmic clouds and particles which become delivered into the oocyte during nurse cell regression.

Protein Families

Pannexin family

Tissue Specificity

In ovary, expressed in inner germarial sheath cells, prefollicular cells, follicle cells, nurse cells and oocytes. Expressed in embryonic epithelial cells. Expressed in foregut and hindgut from stage 11-17, segmentally repeated tracheal placodes at stage

Database Links

KEGG: dme:Dmel_CG4590

STRING: 7227.FBpp0070966

UniGene: Dm.3095

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Inx2 Antibody

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