IPAB Antibody

Datasheet

Code	CSB-PA322404XA01SZB
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: IPAB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40.2 kDa Observed band size: 43 kDa
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Target Background

Product Details

Uniprot No.

P18011

Target Names

IPAB

Alternative Names

Invasin IpaB (62 kDa antigen) ipaB CP0128

Species Reactivity

Shigella flexneri

Immunogen

Recombinant Shigella flexneri IPAB protein (1-312aa)

Immunogen Species

Shigella flexneri

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The IPAB polyclonal antibody is derived from a rabbit administered with a recombinant Shigella flexneri IPAB protein (1-312aa). The serum is collected from the rabbit to obtain polyclonal antibodies, which subsequently undergo protein G affinity chromatography purification. This IPAB antibody is suitable for the detection of Shigella flexneri IPAB protein through ELISA and WB assays.

The Shigella flexneri IPAB protein is a component of the type III secretion system (T3SS) that helps Shigella inject proteins into host cells. These injected proteins manipulate the host cell machinery to allow the bacteria to invade and replicate inside the cell. IPAB is involved in the formation of a translocation pore in the host cell membrane, through which other effector proteins are delivered, ultimately leading to viral invasion and colonization.

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Target Background

Function

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.

Gene References into Functions

IpaB translocon component binds cholesterol with high affinity, cholesterol-dependent association of the translocon with the target cell plasma membrane is essential for translocon activation and effector delivery into mammalian cells PMID: 15819617

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein. Host nucleus. Note=Secreted through the specialized type-III secretion system Mxi/Spa. Inserted into the host cell membrane. Also secreted into the host cell cytoplasm after the escape of bacteria from phagosome, where it colocalizes with ICE. May localize to host cell nucleus during G2/M phase of the host cell cycle.

Protein Families

Invasin protein B family

Database Links

KEGG: sfl:CP0128

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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IPAB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background