ipaB Antibody

Datasheet

Code	CSB-PA322404ZA01SZB
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: IPAB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40.2 kDa Observed band size: 43 kDa
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Product Details

Uniprot No.

P18011

Target Names

ipaB

Alternative Names

Invasin IpaB (62 kDa antigen), ipaB

Species Reactivity

Shigella flexneri

Immunogen

Recombinant Shigella flexneri Invasin ipaB(ipaB),partial (1-312aa)

Immunogen Species

Shigella flexneri

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To generate the ipaB polyclonal antibody, a rabbit is immunized with the recombinant Shigella flexneri invasin ipaB protein (1-312aa). Subsequently, the ipaB antibody is obtained from the rabbit serum and subjected to purification using protein G. This specific ipaB antibody is well-suited for detecting the ipaB protein of Shigella flexneri in ELISA and WB applications.

IpaB is a key component of the type III secretion system (T3SS) of Shigella flexneri, which is responsible for injecting bacterial effector proteins into the host cell. IpaB plays a crucial role in the formation of a pore complex on the host cell membrane, allowing the delivery of effector proteins into the host cell cytoplasm. Once injected, these effector proteins manipulate the host cell machinery to promote bacterial entry and survival. This process helps Shigella flexneri to establish infection and cause diseases such as shigellosis.

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Target Background

Function

Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. Forms a pore with IpaC, which is inserted into the host cell membrane through the Mxi/Spa apparatus, during cell contact. This pore probably allows the translocation of IpaA. IpaB has also been found to be necessary and sufficient to activate macrophage apoptosis by binding to interleukin-1 beta converting enzyme (ICE). Has also been shown to be important, along with IpaD, to block or regulate secretion through the Mxi/Spa translocon in the presence or absence of the secretion signal, respectively. Through interaction with host human MAD2L2, constitutively activates the anaphase-promoting complex APC and induces a cell cycle arrest to prevent epithelial renewal in order to promote bacterial colonization.

Gene References into Functions

IpaB translocon component binds cholesterol with high affinity, cholesterol-dependent association of the translocon with the target cell plasma membrane is essential for translocon activation and effector delivery into mammalian cells PMID: 15819617

Subcellular Location

Secreted. Host cell membrane; Multi-pass membrane protein. Host nucleus. Note=Secreted through the specialized type-III secretion system Mxi/Spa. Inserted into the host cell membrane. Also secreted into the host cell cytoplasm after the escape of bacteria from phagosome, where it colocalizes with ICE. May localize to host cell nucleus during G2/M phase of the host cell cycle.

Protein Families

Invasin protein B family

Database Links

KEGG: sfl:CP0128

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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ipaB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background