ISC1 Antibody

Code CSB-PA340193XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ISC1 Polyclonal antibody
Uniprot No.
Target Names
ISC1
Alternative Names
ISC1 antibody; YER019W antibody; Inositol phosphosphingolipids phospholipase C antibody; IPS phospholipase C antibody; IPS-PLC antibody; EC 3.1.4.- antibody; Neutral sphingomyelinase antibody; N-SMase antibody; nSMase antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ISC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Regulates sphingolipid metabolism in mitochondria, especially the formation of alpha-hydroxylated very long chain phytoceramides. The generated ceramides contribute to the normal function of mitochondria. Also active on sphingomyelin (SM), but this activity is probably not physiologically relevant.
Gene References into Functions
  1. activation of Isc1p in vivo is dependent on PGS1 and on the mitochondrial phospholipids PG/CL PMID: 15611094
  2. results showed that Isc1p contributes specifically to the formation of the C24-, C24:1- and C26-dihydroceramide species PMID: 16201964
  3. Isc1 regulates sphingolipid metabolism in yeast mitochondria. PMID: 17880915
  4. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis. PMID: 18162582
  5. Findings related to the function of ISC1, its localization, mechanisms, and its roles in cell response to different types of stresses. [REVIEW] PMID: 18305313
  6. These results suggest a novel role for Isc1p in allowing the reprogramming of gene expression during the transition from anaerobic to aerobic metabolism. PMID: 19179331

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein.
Protein Families
Neutral sphingomyelinase family
Database Links

KEGG: sce:YER019W

STRING: 4932.YER019W

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