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Recombinant Saccharomyces cerevisiae Inositol phosphosphingolipids phospholipase C (ISC1), partial

Recombinant Saccharomyces cerevisiae Inositol phosphosphingolipids phospholipase C (ISC1), partial

Code	CSB-YP340193SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP340193SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP340193SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP340193SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP340193SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ISC1

Uniprot No.

P40015

Alternative Names

ISC1; YER019W; Inositol phosphosphingolipids phospholipase C; IPS phospholipase C; IPS-PLC; EC 3.1.4.-; Neutral sphingomyelinase; N-SMase; nSMase

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Responsible for the hydrolysis of the phosphosphingolipids (IPS), inositol phosphorylceramide (IPC), mannosylinositol phosphorylceramide (MIPC), and mannosyldiinositol phosphorylceramide (M(IP)2C). Regulates sphingolipid metabolism in mitochondria, especially the formation of alpha-hydroxylated very long chain phytoceramides. The generated ceramides contribute to the normal function of mitochondria. Also active on sphingomyelin (SM), but this activity is probably not physiologically relevant.

Gene References into Functions

activation of Isc1p in vivo is dependent on PGS1 and on the mitochondrial phospholipids PG/CL PMID: 15611094
results showed that Isc1p contributes specifically to the formation of the C24-, C24:1- and C26-dihydroceramide species PMID: 16201964
Isc1 regulates sphingolipid metabolism in yeast mitochondria. PMID: 17880915
These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis. PMID: 18162582
Findings related to the function of ISC1, its localization, mechanisms, and its roles in cell response to different types of stresses. [REVIEW] PMID: 18305313
These results suggest a novel role for Isc1p in allowing the reprogramming of gene expression during the transition from anaerobic to aerobic metabolism. PMID: 19179331