if Antibody

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Code	CSB-PA318925XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) if Polyclonal antibody

Uniprot No.

P12080

Target Names

Alternative Names

if antibody; CG9623 antibody; Integrin alpha-PS2 antibody; Position-specific antigen subunit alpha-2 antibody; Protein inflated) [Cleaved into: Integrin alpha-PS2 heavy chain; Integrin alpha-PS2 light chain] antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) if protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Alpha-PS2/beta-PS is a receptor for Tig, wb and Ten-m. Involved in the function and/or development of the olfactory system.

Gene References into Functions

the conserved transmembrane Drosophila proteoglycan Kon-tiki (Kon, also known as Perdido) interacts with the alphaPS2betaPS integrin (alphaPS2 is encoded by inflated and betaPS by myospheroid) to mediate muscle-tendon adhesion. PMID: 28104814
the integrin heterodimer alphaPS3/betaPS becomes apically enriched and is required for engulfment by the epithelial follicle cells of the Drosophila ovary. PMID: 26398951
Study presents evidence that alphaPS2 and alphaPS3 integrin have different and specific functions in the development of retinal glia PMID: 25731761
Data indicate that Tsp66E and Tsp74F mutant ovaries had elevated inflated gene product alphaPS2 integrin levels and reduced endocytic trafficking. PMID: 23068610
We also demonstrate a role for integrin function in salivary gland migration. PMID: 12729556
analysis of binding by Drosophila alphaPS2betaPS integrin to ligands PMID: 16371365
Alternative splicing in integrin alpha subunit extracellular domains may affect ligand affinity via relatively subtle alterations in integrin conformation. PMID: 17372926
Three mutations that alter different parts of the alphaPS2 beta-propeller, plus a fourth that eliminated a late phase of alphaPS2 expression, all led to a strong decrease in alphaPS2betaPS at muscle ends, but normal levels of talin were recruited. PMID: 17618618
These data suggest a model in which ERK phosphorylation must be spatially coupled to integrin-mediated DIM-7 activation to make a complex that can be imported efficiently. PMID: 17699602

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Subcellular Location

Apical cell membrane; Single-pass type I membrane protein. Lateral cell membrane; Single-pass type I membrane protein. Basal cell membrane; Single-pass type I membrane protein. Note=During mid-oogenesis, localizes to the apical, to the lateral and to the basal membranes of follicle cells. Apical membrane localization peaks at oogenesis stages 9 and 10A in columnar follicle cells overlying the oocyte while decreases in the most posterior follicle cells. During embryogenesis, at the end of germband extension concentrates at the basal membrane of mesodermal cells, where they adhere to the ectoderm. Then, in visceral mesoderm localizes basally, whereas in somatic mesoderm is homogenously distributed.

Protein Families

Integrin alpha chain family

Tissue Specificity

In ovaries, highly expressed in follicle cells. At syncytial blastoderm stage, expressed in the embryonic mesodermal precursors but not in the ectoderm. At embryonic stages 7 and 10, expression is restricted to the mesoderm. At stage 12, expressed in the

Database Links

KEGG: dme:Dmel_CG9623

STRING: 7227.FBpp0074131

UniGene: Dm.7766

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