KAR9 Antibody

Code CSB-PA341330XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAR9 Polyclonal antibody
Uniprot No.
Target Names
KAR9
Alternative Names
KAR9 antibody; YPL269W antibody; Karyogamy protein KAR9 antibody; Cortical protein KAR9 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) KAR9 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in karyogamy. Component of a cortical adaptor complex that orients cytoplasmic microtubules. It may be involved in anchoring cytoplasmic microtubules to the cell cortex.
Gene References into Functions
  1. KAR9 has microtubule end-binding motif LxxPTPh (where x and h represent any and hydrophobic amino acids, respectively). PMID: 28552577
  2. One crystal of Kar9p showed diffraction to 3 A resolution PMID: 23027760
  3. Kar9 is ubiquitylated and degraded by the proteasome after phosphorylation by Cdk1. PMID: 20598539
  4. Kar9p-mediated MT delivery along actin cables does not involve Bud6p at the cell cortex PMID: 15492045
  5. Overproduction of Bim1, but not Kar9, restores Kar9-dependent spindle positioning in the tub4-delta dsyl mutant, reduces cortical dwelling, and promotes Bim1-Kar9 interactions. PMID: 16899509
  6. Three independent mechanisms contribute to Kar9 asymmetry: Cdk1-dependent phosphorylation, sumoylation, and spindle-assembly checkpoint signaling. PMID: 18722122
  7. sumoylation regulates spindle positioning by restricting Kar9p to one spindle pole body. PMID: 18832349

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton. Note=Localizes to the tip of shmoo projections and to the tip of budding cells in a cell-cycle dependent manner.
Database Links

KEGG: sce:YPL269W

STRING: 4932.YPL269W

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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