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Recombinant Saccharomyces cerevisiae Karyogamy protein KAR9 (KAR9), partial

Recombinant Saccharomyces cerevisiae Karyogamy protein KAR9 (KAR9), partial

Code	CSB-YP341330SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP341330SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP341330SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP341330SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP341330SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

KAR9

Uniprot No.

P32526

Alternative Names

KAR9; YPL269W; Karyogamy protein KAR9; Cortical protein KAR9

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in karyogamy. Component of a cortical adaptor complex that orients cytoplasmic microtubules. It may be involved in anchoring cytoplasmic microtubules to the cell cortex.

Gene References into Functions

KAR9 has microtubule end-binding motif LxxPTPh (where x and h represent any and hydrophobic amino acids, respectively). PMID: 28552577
One crystal of Kar9p showed diffraction to 3 A resolution PMID: 23027760
Kar9 is ubiquitylated and degraded by the proteasome after phosphorylation by Cdk1. PMID: 20598539
Kar9p-mediated MT delivery along actin cables does not involve Bud6p at the cell cortex PMID: 15492045
Overproduction of Bim1, but not Kar9, restores Kar9-dependent spindle positioning in the tub4-delta dsyl mutant, reduces cortical dwelling, and promotes Bim1-Kar9 interactions. PMID: 16899509
Three independent mechanisms contribute to Kar9 asymmetry: Cdk1-dependent phosphorylation, sumoylation, and spindle-assembly checkpoint signaling. PMID: 18722122
sumoylation regulates spindle positioning by restricting Kar9p to one spindle pole body. PMID: 18832349