Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) CkIalpha Polyclonal antibody

Uniprot No.

P54367

Target Names

CkIalpha

Alternative Names

CkIalpha antibody; CKI antibody; CG2028 antibody; Casein kinase I isoform alpha antibody; CKI-alpha antibody; DmCK1 antibody; EC 2.7.11.1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) CkIalpha protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Negative regulator of wg signaling. Phosphorylates arm directly or indirectly and stimulates its degradation which prevents inappropriate wg signaling. Phosphorylates smo which promotes its accumulation at the cell surface and its signaling activity in response to hh. Together with dco, regulates proteolytic processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase required for ci processing. Inhibits condensin II interphase activity by promoting degradation of the Cap-H2 regulatory subunit and limiting the levels of chromatin-bound Cap-H2 which regulates interphase chromosome organization.

Gene References into Functions

glucose regulatory role PMID: 25994086
we identify Casein Kinase I alpha (CK1alpha) as an additional negative-regulator of Cap-H2. CK1a-depletion stabilizes Cap-H2 protein and results in an accumulation of Cap-H2 on chromosomes. PMID: 25723539
Expected genes in the Scar-Arp2/3 actin nucleation pathway were identified as well as casein kinase I, which had a similar morphological RNAi signature. PMID: 21041442
CKI(Casein kinase I) functions as a negative regulator of Arm protein, by phosphorylating it on Ser and Thr residues in the N-terminus and targeting it for degradation. PMID: 11927557
2 proteins reported to function in Wingless(Wg) signaling identified as Hh pathway components: Dally-like protein required for Hh signal reception, and casein kinase 1alpha, a candidate tumor suppressor that regulates activities of both Hh and Wg pathways PMID: 12663920
expression of doubletime in Drosophila S2 cells produced a CKI-7-sensitive kinase activity which was reduced by both the dbt(S) and dbt(L) mutations of circadian rhythm PMID: 14701759
doubletime and CKII kinases collaborate to potentiate Drosophila PER transcriptional repressor activity PMID: 14759367
Several CKI isoforms including CKIalpha and Gish/CKIgamma can phosphorylate the Wg coreceptor Arrow (Arr), which may account, at least in part, for their positive roles in the Wg pathway. PMID: 16987508

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily

Database Links

KEGG: dme:Dmel_CG2028

STRING: 7227.FBpp0073513

UniGene: Dm.2166

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA006065XA01DLU
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

CkIalpha Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background