TPK1 Antibody

Code CSB-PA814494XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) TPK1 Polyclonal antibody
Uniprot No.
Target Names
TPK1
Alternative Names
TPK1; KCO1; At5g55630; MDF20.7; Two-pore potassium channel 1; AtTPK1; Calcium-activated outward-rectifying potassium channel 1; AtKCO1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana TPK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Voltage-independent, large conductance and potassium-selective tonoplast ion channel. Regulated by cytoplasmic calcium and pH. Does not mediate slow-vacuolar (SV) ionic currents, but essential to establish VK currents. Has some permeability for Rb(+) and NH(4)(+), but none for Na(+), Cs(+) or Li(+). Involved in intracellular K(+) redistribution and/or K(+) retranslocation between different tissues.
Gene References into Functions
  1. ATKCO1 can form functional potassium transport systems in E. coli PMID: 23656881
  2. TPK1 must assemble correctly to pass endoplasmic reticulum quality control. PMID: 21697507
  3. data clearly demonstrate that TPK1 (KCO1) is a voltage-independent, Ca(2+)-activated, K(+)-selective ion channel in the vacuolar membrane that does not mediate SV-type ionic currents [TPK1] PMID: 16113216
  4. The two-pore channel TPK1 gene encodes the vacuolar K+ conductance and plays a role in K+ homeostasis. PMID: 17563365
  5. findings show that AtTPK1 (KCO1), interacts with 14-3-3 proteins (general regulating factors, GRFs; TPK1 seems to provide for a Ca(2+)- and 14-3-3-sensitive mechanism capable of controlling cytoplasmic potassium homeostasis in plants PMID: 17764516

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Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Note=Tonoplast.
Protein Families
Two pore domain potassium channel (TC 1.A.1.7) family
Tissue Specificity
Detected in mesophyll cells, guard cells and vascular tissues of the leaves. Expressed in the hilum, where the funiculus is attached during fruit maturation and in the embryo. Also expressed at a lower level in seedlings, root tips and elongation zones, a
Database Links

KEGG: ath:AT5G55630

STRING: 3702.AT5G55630.1

UniGene: At.20254

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