Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) KIN11 Polyclonal antibody

Uniprot No.

P92958

Target Names

KIN11

Alternative Names

KIN11 antibody; AKIN11 antibody; SNR1 antibody; SNRK1.2 antibody; At3g29160 antibody; MXE2.16 antibody; SNF1-related protein kinase catalytic subunit alpha KIN11 antibody; AKIN11 antibody; EC 2.7.11.1 antibody; AKIN alpha-1 antibody; AKINalpha1 antibody; SNF1-related kinase 1.2 antibody; SnRK1.2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana KIN11 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, a central regulator of cellular energy homeostasis, which, in response to seemingly unrelated darkness, sugar and stress conditions, activates energy-producing pathways and inhibits energy-consuming processes. May play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN11 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Involved in innate antiviral defenses. Phosphorylates REM4.1 in vitro. Phosphorylates ADK2 in vitro.

Gene References into Functions

Studies indicate that protein-serine-threonine kinase SnRK1 affects metabolic adaptation, including plastidial and mitochondrial functions [Review]. PMID: 29311271
SNRK1.2 (At3g29160) role in regulating metabolism and transcription in response to cellular energy status. PMID: 29114081
Transcription activation function of AC2 and the inactivation of SnRK1.2 induced the transcriptome changes in Arabidopsis. PMID: 25403083
SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. [SNRK1.2] PMID: 22232383
SnR2.8 might be involved in a parallel pathway for activation of AREB/ABF bZIP-type transcription factors in response to drought stress. PMID: 20375108
SRK2C is a positive regulator of drought tolerance in Arabidopsis roots.drought tolerance coincided with up-regulation of many stress-responsive genes, for example, RD29A, COR15A, and DREB1A/CBF3. PMID: 15561775

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Subcellular Location

Plastid, chloroplast. Cytoplasm. Endoplasmic reticulum.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily

Tissue Specificity

Expressed in roots, shoots, flower buds, flowers, siliques and leaves. Restrictly expressed to the base of the leaf, the vascular tissue, and the hydathodes.

Database Links

KEGG: ath:AT3G29160

STRING: 3702.AT3G29160.1

UniGene: At.184

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KIN11 Antibody

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