Recombinant Arabidopsis thaliana SNF1-related protein kinase catalytic subunit alpha KIN11 (KIN11), partial

Code CSB-YP310102DOA
MSDS
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Source Yeast
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Code CSB-EP310102DOA
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Source E.coli
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Code CSB-EP310102DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP310102DOA
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Source Baculovirus
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Code CSB-MP310102DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIN11
Uniprot No.
Alternative Names
KIN11; AKIN11; SNR1; SNRK1.2; At3g29160; MXE2.16; SNF1-related protein kinase catalytic subunit alpha KIN11; AKIN11; EC 2.7.11.1; AKIN alpha-1; AKINalpha1; SNF1-related kinase 1.2; SnRK1.2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, a central regulator of cellular energy homeostasis, which, in response to seemingly unrelated darkness, sugar and stress conditions, activates energy-producing pathways and inhibits energy-consuming processes. May play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN11 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Involved in innate antiviral defenses. Phosphorylates REM4.1 in vitro. Phosphorylates ADK2 in vitro.
Gene References into Functions
  1. Studies indicate that protein-serine-threonine kinase SnRK1 affects metabolic adaptation, including plastidial and mitochondrial functions [Review]. PMID: 29311271
  2. SNRK1.2 (At3g29160) role in regulating metabolism and transcription in response to cellular energy status. PMID: 29114081
  3. Transcription activation function of AC2 and the inactivation of SnRK1.2 induced the transcriptome changes in Arabidopsis. PMID: 25403083
  4. SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. [SNRK1.2] PMID: 22232383
  5. SnR2.8 might be involved in a parallel pathway for activation of AREB/ABF bZIP-type transcription factors in response to drought stress. PMID: 20375108
  6. SRK2C is a positive regulator of drought tolerance in Arabidopsis roots.drought tolerance coincided with up-regulation of many stress-responsive genes, for example, RD29A, COR15A, and DREB1A/CBF3. PMID: 15561775

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Subcellular Location
Plastid, chloroplast. Cytoplasm. Endoplasmic reticulum.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Expressed in roots, shoots, flower buds, flowers, siliques and leaves. Restrictly expressed to the base of the leaf, the vascular tissue, and the hydathodes.
Database Links

KEGG: ath:AT3G29160

STRING: 3702.AT3G29160.1

UniGene: At.184

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