Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Klp61F Polyclonal antibody

Uniprot No.

P46863

Target Names

Klp61F

Alternative Names

Klp61F antibody; KLP2 antibody; CG9191 antibody; Kinesin-like protein Klp61F antibody; Bipolar kinesin KRP-130 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Klp61F protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function. Forms cross-links between microtubules within interpolar microtubule bundles. Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Displays microtubule-stimulated ATPase activity. Required for normal fusome organization. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.

Gene References into Functions

KLP61F is required for spindle and centromere symmetry in oocytes. PMID: 27932541
Kinesin-5 pauses at microtubule plus ends and enhances polymerization by stabilizing longitudinal tubulin-tubulin interactions. These activities underlie the ability of kinesin-5 to slide and stabilize microtubule bundles in cells. PMID: 26437877
Klp61F knockdown inhibits post-Golgi transport in Drosophila S2 cells. PMID: 23857769
A staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments. PMID: 23299893
Tyrosines in the kinesin-5 (KLP61F)head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity. PMID: 19800237
KLP61F prevents Ncd-mediated collapse of a nonmicrotubule matrix derived from the interphase nucleus PMID: 15367580
Kinesin-5 motors can function by cross-linking and sliding adjacent spindle microtubules without the need for a static spindle matrix. PMID: 18678711
results suggest that during morphogenesis of the embryo mitotic spindle, KLP61F's crosslinking & sliding activities could facilitate accumulation of KLP61F within antiparallel interpolar microtubules at the equator PMID: 19062285
KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates PMID: 19158379
calculated the structure of a K5 motor bound to MTs at 9 A resolution, providing insight into this important interaction. PMID: 19285086
The work tends to support the "sliding filament" mechanism for KLP61F action in Drosophila embryo spindles. PMID: 19291760

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Subcellular Location

Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily

Database Links

KEGG: dme:Dmel_CG9191

STRING: 7227.FBpp0072616

UniGene: Dm.804

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Klp61F Antibody

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