Recombinant Drosophila melanogaster Bipolar kinesin KRP-130 (Klp61F), partial

Code CSB-YP342037DLU
MSDS
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Source Yeast
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Code CSB-EP342037DLU
MSDS
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Source E.coli
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Code CSB-EP342037DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342037DLU
MSDS
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Source Baculovirus
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Code CSB-MP342037DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Klp61F
Uniprot No.
Alternative Names
Klp61F; KLP2; CG9191; Kinesin-like protein Klp61F; Bipolar kinesin KRP-130
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function. Forms cross-links between microtubules within interpolar microtubule bundles. Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Displays microtubule-stimulated ATPase activity. Required for normal fusome organization. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Gene References into Functions
  1. KLP61F is required for spindle and centromere symmetry in oocytes. PMID: 27932541
  2. Kinesin-5 pauses at microtubule plus ends and enhances polymerization by stabilizing longitudinal tubulin-tubulin interactions. These activities underlie the ability of kinesin-5 to slide and stabilize microtubule bundles in cells. PMID: 26437877
  3. Klp61F knockdown inhibits post-Golgi transport in Drosophila S2 cells. PMID: 23857769
  4. A staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments. PMID: 23299893
  5. Tyrosines in the kinesin-5 (KLP61F)head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity. PMID: 19800237
  6. KLP61F prevents Ncd-mediated collapse of a nonmicrotubule matrix derived from the interphase nucleus PMID: 15367580
  7. Kinesin-5 motors can function by cross-linking and sliding adjacent spindle microtubules without the need for a static spindle matrix. PMID: 18678711
  8. results suggest that during morphogenesis of the embryo mitotic spindle, KLP61F's crosslinking & sliding activities could facilitate accumulation of KLP61F within antiparallel interpolar microtubules at the equator PMID: 19062285
  9. KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates PMID: 19158379
  10. calculated the structure of a K5 motor bound to MTs at 9 A resolution, providing insight into this important interaction. PMID: 19285086
  11. The work tends to support the "sliding filament" mechanism for KLP61F action in Drosophila embryo spindles. PMID: 19291760

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Subcellular Location
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
Database Links

KEGG: dme:Dmel_CG9191

STRING: 7227.FBpp0072616

UniGene: Dm.804

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