Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YKU70 Polyclonal antibody

Uniprot No.

P32807

Target Names

YKU70

Alternative Names

YKU70 antibody; HDF1 antibody; NES24 antibody; YMR284W antibody; YM8021.10ATP-dependent DNA helicase II subunit 1 antibody; EC 3.6.4.12 antibody; ATP-dependent DNA helicase II subunit Ku70 antibody; High affinity DNA-binding factor subunit 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) YKU70 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.

Gene References into Functions

We found that the yku70-R456E mutant strain, which has markedly reduced association of Ku at telomeres, maintained WT levels of TLC1 nuclear localization. PMID: 24879463
Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku PMID: 24567323
The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. [YKU70] PMID: 21441303
Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. PMID: 20729809
Ku inhibits Exo1-dependent double-strand break end resection in the absence of the MRX complex in vitro. PMID: 20834227

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Subcellular Location

Nucleus. Chromosome, telomere.

Protein Families

Ku70 family

Database Links

KEGG: sce:YMR284W

STRING: 4932.YMR284W

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YKU70 Antibody

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