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Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase II subunit 1 (YKU70), partial

Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase II subunit 1 (YKU70), partial

Code	CSB-YP339296SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP339296SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP339296SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP339296SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP339296SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

YKU70

Uniprot No.

P32807

Alternative Names

YKU70; HDF1; NES24; YMR284W; YM8021.10ATP-dependent DNA helicase II subunit 1; EC 3.6.4.12; ATP-dependent DNA helicase II subunit Ku70; High affinity DNA-binding factor subunit 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.

Gene References into Functions

We found that the yku70-R456E mutant strain, which has markedly reduced association of Ku at telomeres, maintained WT levels of TLC1 nuclear localization. PMID: 24879463
Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku PMID: 24567323
The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation. [YKU70] PMID: 21441303
Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. PMID: 20729809
Ku inhibits Exo1-dependent double-strand break end resection in the absence of the MRX complex in vitro. PMID: 20834227

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Subcellular Location

Nucleus. Chromosome, telomere.

Protein Families

Ku70 family

Database Links

KEGG: sce:YMR284W

STRING: 4932.YMR284W

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