Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Lats1 Polyclonal antibody

Uniprot No.

Q8BYR2

Target Names

Lats1

Alternative Names

Lats1 antibody; WartsSerine/threonine-protein kinase LATS1 antibody; EC 2.7.11.1 antibody; Large tumor suppressor homolog 1 antibody; WARTS protein kinase antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Lats1 protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.

Gene References into Functions

disruption of TAZ/YAP activity alleviates tumor burden in Lats1/2-deficient mice and inhibits human malignant peripheral nerve sheath tumors cell proliferation PMID: 29438698
Results show that tripterygium glycosides suppress endogenous Lats1 expression in ovarian granulosa cells by stimulating miR-15a expression which directly targets the 3'UTR of Lats1, induces cytotoxicity of ovarian granulosa cells, and ultimately cause premature ovarian failure. PMID: 30092342
During and after liver development, the activation of YAP/TAZ induced by loss of Lats1/2 forces hepatoblasts or hepatocytes to commit to the biliary epithelial cell lineage. PMID: 27358050
Epicardial Deletion of Lats1 gene Results in Defective Coronary Vessel Development. PMID: 29689192
Collectively, these data indicate that Yap-induced Epiregulin signaling promotes the identity of submandibular gland ductal progenitors and that removal of nuclear Yap by Lats1/2-mediated signaling is critical for proper ductal maturation. PMID: 28492365
Study demonstrates that, in 3 different murine syngeneic tumor models (B16, SCC7, and 4T1), loss of the Hippo pathway kinases LATS1/2 (large tumor suppressor 1 and 2) in tumor cells inhibits tumor growth; data indicate a new paradigm for how tumor immunogenicity is regulated through the Hippo signaling pathway in tumor cells. PMID: 27912060
Results showed that the Hippo pathway is active in ovarian follicles and that LATS1 is required to maintain the pool of germ cells and primordial follicles. PMID: 26040669
The control of LATS activation by angiotensin II and subsequent YAP localization is important for podocyte homeostasis and survival. PMID: 25393475
Willin is predominantly expressed in fibroblasts and that Willin expression activates the Hippo signaling cascade PMID: 23593160
LATS1 tumor suppressor is a novel actin-binding protein and negative regulator of actin polymerization. PMID: 21808298
KIBRA associates with and activates Lats (large tumor suppressor) 1 and 2 kinases by stimulating their phosphorylation on the hydrophobic motif PMID: 21233212
Lats1 enhances the activity of FOXl2 as a repressor of the StAr promoter. st PMID: 20407010
Overexpression of YAP2 in cells promoted apoptosis, whereas the Mst2/Lats1-induced phosphorylation of YAP partially rescued the cells from apoptotic death. PMID: 18640976
Genome-wide location arrays in cell lines of various cell types revealed that Lats1 was a transcriptional target of CUX1. PMID: 19656388

Hide All

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family

Database Links

KEGG: mmu:16798

STRING: 10090.ENSMUSP00000041915

UniGene: Mm.34083

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Lats1 Antibody

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