LBD16 Antibody

Code CSB-PA865924XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LBD16 Polyclonal antibody
Uniprot No.
Target Names
LBD16
Alternative Names
LBD16 antibody; ASL18 antibody; At2g42430 antibody; MHK10.15LOB domain-containing protein 16 antibody; ASYMMETRIC LEAVES 2-like protein 18 antibody; AS2-like protein 18 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LBD16 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional activator. Involved in lateral root formation. Regulated by the transcriptional activators ARF7 and ARF19. Functions in the initiation and emergence of lateral roots, in conjunction with LBD18, downstream of ARF7 and ARF19. Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development.
Gene References into Functions
  1. conserved Leu or Val residues in the coiled-coil motifs of LBD16 and LBD18 are critical for their dimerization as well as the transcriptional regulation to display their biological functions. PMID: 29227192
  2. the WOX11-LBD16 pathway promotes pluripotency acquisition in callus cells. PMID: 29361138
  3. Data show that the conserved Leu or valine residues in the coiled-coil motif are critical for the dimerization of LBD16 or LBD18 transcription factors. PMID: 28336771
  4. LBD16 is a key molecular player in the xylem pole pericycle cells during giant cells and gall development. PMID: 24803293
  5. PUCHI co-acts with LBD16 and LBD18 to control lateral root primordium development and lateral root emergence. PMID: 23749813
  6. LBD16, LBD29, and LBD18 are involved in cell cycle progression of the pericycle in response to auxin. PMID: 22699776
  7. Data show that overexpression of each of the LBD genes promotes callus formation in the absence of exogenous phytohormones, while suppression of LBD function inhibits the callus induction process. PMID: 22508267
  8. Results indicate that the localized activity of LBD16/ASL18 and its related LBD/ASLs is involved in the symmetry breaking of lateral root (LR) founder cells for LR initiation, a key step for constructing the plant root system. PMID: 22278921
  9. LBD16 and LBD18 might function in a combinatorial manner in lateral root formation. PMID: 19717544

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Subcellular Location
Nucleus.
Protein Families
LOB domain-containing protein family
Tissue Specificity
Expressed in roots and faintly in shoots.
Database Links

KEGG: ath:AT2G42430

STRING: 3702.AT2G42430.1

UniGene: At.13375

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