Recombinant Arabidopsis thaliana LOB domain-containing protein 16 (LBD16)

Code CSB-YP865924DOA
MSDS
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Source Yeast
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Code CSB-EP865924DOA
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Source E.coli
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Code CSB-EP865924DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865924DOA
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Source Baculovirus
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Code CSB-MP865924DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LBD16
Uniprot No.
Alternative Names
LBD16; ASL18; At2g42430; MHK10.15LOB domain-containing protein 16; ASYMMETRIC LEAVES 2-like protein 18; AS2-like protein 18
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-245
Target Protein Sequence
MASSGNGTTA GTGSPCGACK FLRRKCASDC IFAPYFSSEQ GAARFAAIHK VFGASNVSKL LLNVPIHDRC EAVVTIAYEA QARLHDPVYG CVSHIFALQQ QVAFLQSQVM QMKAQIAGHQ TSAAGDLRHS SESTNQFMTW QQTSVSPIGS AYSTPYNHHQ PYYGHVNPNN PVSPQSSLEE SFSNTSSDVT TTANVRETHH QTGGGVYGHD GIGFHEGYPN KKRSVSYCSS DLGELQALAL RMMKN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator. Involved in lateral root formation. Regulated by the transcriptional activators ARF7 and ARF19. Functions in the initiation and emergence of lateral roots, in conjunction with LBD18, downstream of ARF7 and ARF19. Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development.
Gene References into Functions
  1. conserved Leu or Val residues in the coiled-coil motifs of LBD16 and LBD18 are critical for their dimerization as well as the transcriptional regulation to display their biological functions. PMID: 29227192
  2. the WOX11-LBD16 pathway promotes pluripotency acquisition in callus cells. PMID: 29361138
  3. Data show that the conserved Leu or valine residues in the coiled-coil motif are critical for the dimerization of LBD16 or LBD18 transcription factors. PMID: 28336771
  4. LBD16 is a key molecular player in the xylem pole pericycle cells during giant cells and gall development. PMID: 24803293
  5. PUCHI co-acts with LBD16 and LBD18 to control lateral root primordium development and lateral root emergence. PMID: 23749813
  6. LBD16, LBD29, and LBD18 are involved in cell cycle progression of the pericycle in response to auxin. PMID: 22699776
  7. Data show that overexpression of each of the LBD genes promotes callus formation in the absence of exogenous phytohormones, while suppression of LBD function inhibits the callus induction process. PMID: 22508267
  8. Results indicate that the localized activity of LBD16/ASL18 and its related LBD/ASLs is involved in the symmetry breaking of lateral root (LR) founder cells for LR initiation, a key step for constructing the plant root system. PMID: 22278921
  9. LBD16 and LBD18 might function in a combinatorial manner in lateral root formation. PMID: 19717544

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Subcellular Location
Nucleus.
Protein Families
LOB domain-containing protein family
Tissue Specificity
Expressed in roots and faintly in shoots.
Database Links

KEGG: ath:AT2G42430

STRING: 3702.AT2G42430.1

UniGene: At.13375

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