Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LBD18 Polyclonal antibody

Uniprot No.

O22131

Target Names

LBD18

Alternative Names

LBD18 antibody; ASL20 antibody; At2g45420 antibody; F4L23.7 antibody; LOB domain-containing protein 18 antibody; ASYMMETRIC LEAVES 2-like protein 20 antibody; AS2-like protein 20 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana LBD18 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in the positive regulation of tracheary element (TE) differentiation. Involved in a positive feedback loop that maintains or promotes NAC030/VND7 expression that regulates TE differentiation-related genes. Functions in the initiation and emergence of lateral roots, in conjunction with LBD16, downstream of ARF7 and ARF19. Transcriptional activator that directly regulates EXPA14, a gene encoding a cell wall-loosening factor that promotes lateral root emergence. Activates EXPA14 by directly binding to a specific region of its promoter. Transcriptional activator that directly regulates EXPA17, a gene encoding a cell wall-loosening factor that promotes lateral root emergence. Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development.

Gene References into Functions

This study revealed the molecular function of LBD18 as a specific DNA-binding transcription factor that activates EXP14 expression by directly binding to its promoter. PMID: 22974309
GBF INTERACTING PROTEIN1 (GIP1) interacts with LBD18 in yeast and Arabidopsis protoplasts. Reverse-transcription-pcr analysis showed overlapping expression of GIP1 and LBD18 in roots, aerial parts, and rosette leaves of Arabidopsis. PMID: 24484953
Results suggest that LBD18 up-regulates EXPA17 to enhance cell separation to promote lateral roots (LRs) emergence in Arabidopsis. PMID: 23872272
the proline mutation completely abolished LBD18 DNA-binding activity. PMID: 23430048
E2Fa expression is regulated by the LATERAL ORGAN BOUNDARY DOMAIN18/LATERAL ORGAN BOUNDARY DOMAIN33 (LBD18/LBD33) dimer that is, in turn, regulated by the auxin signaling pathway. PMID: 22003076

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Subcellular Location

Nucleus.

Protein Families

LOB domain-containing protein family

Tissue Specificity

Expressed in roots, stems, leaves and flowers. Expressed in vascular tissues of hypocotyls, leaves, roots, developing floral organs and siliques.

Database Links

KEGG: ath:AT2G45420

STRING: 3702.AT2G45420.1

UniGene: At.21365

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LBD18 Antibody

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