Recombinant Arabidopsis thaliana LOB domain-containing protein 18 (LBD18)

Code CSB-YP522770DOA
MSDS
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Source Yeast
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Code CSB-EP522770DOA
MSDS
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Source E.coli
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Code CSB-EP522770DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522770DOA
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Source Baculovirus
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Code CSB-MP522770DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LBD18
Uniprot No.
Alternative Names
LBD18; ASL20; At2g45420; F4L23.7; LOB domain-containing protein 18; ASYMMETRIC LEAVES 2-like protein 20; AS2-like protein 20
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-262
Target Protein Sequence
MSGGGNTITA VGGGGGGCGG GGSSGGGGSS GGGGGGPCGA CKFLRRKCVP GCIFAPYFDS EQGSAYFAAV HKVFGASNVS KLLLHIPVHR RSDAVVTICY EAQARIRDPI YGCVAHIFAL QQQVVNLQAE VSYLQAHLAS LELPQPQTRP QPMPQPQPLF FTPPPPLAIT DLPASVSPLP STYDLASIFD QTTSSSAWAT QQRRFIDPRH QYGVSSSSSS VAVGLGGENS HDLQALAHEL LHRQGSPPPA ATDHSPSRTM SR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the positive regulation of tracheary element (TE) differentiation. Involved in a positive feedback loop that maintains or promotes NAC030/VND7 expression that regulates TE differentiation-related genes. Functions in the initiation and emergence of lateral roots, in conjunction with LBD16, downstream of ARF7 and ARF19. Transcriptional activator that directly regulates EXPA14, a gene encoding a cell wall-loosening factor that promotes lateral root emergence. Activates EXPA14 by directly binding to a specific region of its promoter. Transcriptional activator that directly regulates EXPA17, a gene encoding a cell wall-loosening factor that promotes lateral root emergence. Acts downstream of the auxin influx carriers AUX1 and LAX1 in the regulation of lateral root initiation and development.
Gene References into Functions
  1. This study revealed the molecular function of LBD18 as a specific DNA-binding transcription factor that activates EXP14 expression by directly binding to its promoter. PMID: 22974309
  2. GBF INTERACTING PROTEIN1 (GIP1) interacts with LBD18 in yeast and Arabidopsis protoplasts. Reverse-transcription-pcr analysis showed overlapping expression of GIP1 and LBD18 in roots, aerial parts, and rosette leaves of Arabidopsis. PMID: 24484953
  3. Results suggest that LBD18 up-regulates EXPA17 to enhance cell separation to promote lateral roots (LRs) emergence in Arabidopsis. PMID: 23872272
  4. the proline mutation completely abolished LBD18 DNA-binding activity. PMID: 23430048
  5. E2Fa expression is regulated by the LATERAL ORGAN BOUNDARY DOMAIN18/LATERAL ORGAN BOUNDARY DOMAIN33 (LBD18/LBD33) dimer that is, in turn, regulated by the auxin signaling pathway. PMID: 22003076

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Subcellular Location
Nucleus.
Protein Families
LOB domain-containing protein family
Tissue Specificity
Expressed in roots, stems, leaves and flowers. Expressed in vascular tissues of hypocotyls, leaves, roots, developing floral organs and siliques.
Database Links

KEGG: ath:AT2G45420

STRING: 3702.AT2G45420.1

UniGene: At.21365

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