Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AS2 Polyclonal antibody

Uniprot No.

O04479

Target Names

AS2

Alternative Names

AS2 antibody; LBD6 antibody; At1g65620 antibody; F5I14.15Protein ASYMMETRIC LEAVES 2 antibody; LOB domain-containing protein 6 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AS2 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Negative regulator of cell proliferation in the adaxial side of leaves. Regulates the formation of a symmetric lamina and the establishment of venation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 1 (AS1) to repress the knox homeobox genes KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis. Required for the binding of AS1 to the KNOX genes. Involved in leaf polarity establishment by functioning cooperatively with RH10 or RID2 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures.

Gene References into Functions

AS2 role in the DNA methylation and leaf polarity.AS2 binds specifically the sequence containing CpGs in exon 1 of ARF3. PMID: 29415182
ASYMMETRIC LEAVES2 expression is critical for leaf axial patterning. PMID: 24006428
These results suggest that AS2 bodies function to properly distribute AS2 to daughter cells during cell division in leaf primordia; and this process is controlled at least partially by signals encoded by the AS2 sequence itself. PMID: 22351044
AS2 and the miRNA319-targeted TCP transcription factors form protein complexes, which associate with promoter regions and downregulate BP and KNAT2 expression. PMID: 22380849
AS2 might be involved in the determination of abaxial polarity in cockscomb plants. PMID: 22143880
AS2 genes are important for repressing class I KNOTTED1-like homeobox (KNOX) genes and specifying leaf adaxial identity in leaf development PMID: 16006579
Results suggest that histone deacetylases HDT1/HD2A and HDT2/HD2B, and ASYMMETRIC LEAVES (AS) 1 and 2 act independently to control levels and/or patterns of miR165/166 distribution and the development of adaxial-abaxial leaf polarity. PMID: 17293570
AS2 represses cell proliferation in the adaxial domain in the presence of asymmetric leaves 1, and adaxial expression of AS2 at an appropriate level is critical for the development of a symmetrical, expanded lamina. PMID: 17559509

Hide All

Subcellular Location

Nucleus.

Protein Families

LOB domain-containing protein family

Tissue Specificity

Expressed in young shoots, roots, stems, leaves, flowers and adaxial domains of cotyledonary and leaves primordia.

Database Links

KEGG: ath:AT1G65620

STRING: 3702.AT1G65620.1

UniGene: At.27203

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA517324XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

AS2 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background