let-99 Antibody

Code CSB-PA632409XA01CXY
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans let-99 Polyclonal antibody
Uniprot No.
Target Names
let-99
Alternative Names
let-99 antibody; K08E7.3Protein let-99 antibody; Lethal protein 99 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans let-99 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Required for the proper orientation of spindles after the establishment of polarity. May play a role in interactions between the astral microtubules and the cortical cytoskeleton. Required for asymmetric forces on nuclei and spindles. Acts downstream of the PAR signaling as an intermediate that transduces polarity information to the machinery that positions the mitotic spindle, possibly by regulating force generation. Regulates gpr-1/2 asymmetric cortical localization during the first embryonic cell divisions. Acts antagonistically to the gpr-1/2 signaling pathway. Regulates mes-1 expression and/or localization pattern during early embryogenesis.
Gene References into Functions
  1. LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells. PMID: 28701343
  2. LET-99 acts in the Mes-1/Src-1 pathway for mitotic spindle positioning. PMID: 27672093
  3. The DEP domain protein LET-99 acts upstream of the heterotrimeric G protein alpha subunit GPA-11 to control activation of the stress kinase JNK-1. PMID: 25064737
  4. LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos PMID: 20421425
Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Database Links

KEGG: cel:CELE_K08E7.3

STRING: 6239.K08E7.3

UniGene: Cel.6064

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1