Recombinant Protein let-99 (let-99), partial

Code CSB-YP632409CXY
MSDS
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Source Yeast
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Code CSB-EP632409CXY
MSDS
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Source E.coli
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Code CSB-EP632409CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP632409CXY
MSDS
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Source Baculovirus
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Code CSB-MP632409CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
let-99
Uniprot No.
Alternative Names
let-99; K08E7.3Protein let-99; Lethal protein 99
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the proper orientation of spindles after the establishment of polarity. May play a role in interactions between the astral microtubules and the cortical cytoskeleton. Required for asymmetric forces on nuclei and spindles. Acts downstream of the PAR signaling as an intermediate that transduces polarity information to the machinery that positions the mitotic spindle, possibly by regulating force generation. Regulates gpr-1/2 asymmetric cortical localization during the first embryonic cell divisions. Acts antagonistically to the gpr-1/2 signaling pathway. Regulates mes-1 expression and/or localization pattern during early embryogenesis.
Gene References into Functions
  1. LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the contractile ring. These and other results suggest a positive feedback model in which LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin. LET-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically dividing cells. PMID: 28701343
  2. LET-99 acts in the Mes-1/Src-1 pathway for mitotic spindle positioning. PMID: 27672093
  3. The DEP domain protein LET-99 acts upstream of the heterotrimeric G protein alpha subunit GPA-11 to control activation of the stress kinase JNK-1. PMID: 25064737
  4. LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos PMID: 20421425
Subcellular Location
Cytoplasm. Cytoplasm, cell cortex.
Database Links

KEGG: cel:CELE_K08E7.3

STRING: 6239.K08E7.3

UniGene: Cel.6064

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