LHT1 Antibody

Code CSB-PA872267XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) LHT1 Polyclonal antibody
Uniprot No.
Target Names
LHT1
Alternative Names
LHT1 antibody; At5g40780 antibody; K1B16.3 antibody; Lysine histidine transporter 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana LHT1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Amino acid-proton symporter. Transporter with a broad specificity for histidine, lysine, glutamic acid, alanine, serine, proline and glycine. Involved in both apoplastic transport of amino acids in leaves and their uptake by roots.
Gene References into Functions
  1. Seedlings deficient in LHT1 exhibited a reduction with respect to both the uptake and root-to-shoot transfer of chlorantraniliprole-glycine conjugate PMID: 30403859
  2. LHT1 expression is crucial for plant uptake of organic Nitrogen from soil. PMID: 27943312
  3. A class of amino acid transporters including LHT1 takes part in transport of 1-Aminocyclopropane-1-carboxylic acid (ACC), thereby influencing exogenous ACC-induced ethylene responses in A. thaliana.[LHT1] PMID: 25520403
  4. Map-based cloning of ARE2 demonstrated that LYSINE HISTIDINE TRANSPORTER1 (LHT1), encoding an amino acid transporter, is the gene responsible. PMID: 25520403
  5. LHT1 functions in the uptake of amino acids in roots and the supply of leaf mesophyll with xylem-derived amino acids. PMID: 16816136

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Amino acid/polyamine transporter 2 family, Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily
Tissue Specificity
Expressed in roots, stems, flowers, leaves, siliques and pollen. Found in the tips of roots and in the rhizodermis of emerging roots and in lateral roots. Higher expression in older leaves as compared to joung leaves. Detected first at the hydathodes, the
Database Links

KEGG: ath:AT5G40780

STRING: 3702.AT5G40780.1

UniGene: At.10818

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