Recombinant Arabidopsis thaliana Lysine histidine transporter 1 (LHT1), partial

Code CSB-YP872267DOA
MSDS
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Source Yeast
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Code CSB-EP872267DOA
MSDS
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Source E.coli
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Code CSB-EP872267DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872267DOA
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Source Baculovirus
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Code CSB-MP872267DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LHT1
Uniprot No.
Alternative Names
LHT1; At5g40780; K1B16.3; Lysine histidine transporter 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Amino acid-proton symporter. Transporter with a broad specificity for histidine, lysine, glutamic acid, alanine, serine, proline and glycine. Involved in both apoplastic transport of amino acids in leaves and their uptake by roots.
Gene References into Functions
  1. Seedlings deficient in LHT1 exhibited a reduction with respect to both the uptake and root-to-shoot transfer of chlorantraniliprole-glycine conjugate PMID: 30403859
  2. LHT1 expression is crucial for plant uptake of organic Nitrogen from soil. PMID: 27943312
  3. A class of amino acid transporters including LHT1 takes part in transport of 1-Aminocyclopropane-1-carboxylic acid (ACC), thereby influencing exogenous ACC-induced ethylene responses in A. thaliana.[LHT1] PMID: 25520403
  4. Map-based cloning of ARE2 demonstrated that LYSINE HISTIDINE TRANSPORTER1 (LHT1), encoding an amino acid transporter, is the gene responsible. PMID: 25520403
  5. LHT1 functions in the uptake of amino acids in roots and the supply of leaf mesophyll with xylem-derived amino acids. PMID: 16816136

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Amino acid/polyamine transporter 2 family, Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily
Tissue Specificity
Expressed in roots, stems, flowers, leaves, siliques and pollen. Found in the tips of roots and in the rhizodermis of emerging roots and in lateral roots. Higher expression in older leaves as compared to joung leaves. Detected first at the hydathodes, the
Database Links

KEGG: ath:AT5G40780

STRING: 3702.AT5G40780.1

UniGene: At.10818

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