Lima1 Antibody

Code CSB-PA887660XA01MO
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Product Details

Full Product Name
Rabbit anti-Mus musculus (Mouse) Lima1 Polyclonal antibody
Uniprot No.
Target Names
Lima1
Alternative Names
Lima1 antibody; D15Ertd366e antibody; EplinLIM domain and actin-binding protein 1 antibody; Epithelial protein lost in neoplasm antibody; mEPLIN antibody
Raised in
Rabbit
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) Lima1 protein
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake.
Gene References into Functions
  1. this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption. PMID: 29880681
  2. findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN. PMID: 25609703
  3. Eplin-alpha transcription is regulated by actin-MAL-SRF signalling. PMID: 20236507
  4. EPLIN may function in growth control by associating with and regulating the actin cytoskeleton PMID: 11950948
  5. ERK-mediated phosphorylation of EPLIN contributes to actin filament reorganization and enhanced cell motility. PMID: 17875928
  6. EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion PMID: 18093941

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell membrane. Cell projection, ruffle.
Tissue Specificity
Highly expressed in the small intestine, including the duodenum, jejunum, and ileum. Low expression in the liver and very low expressed in the heart, spleen, lung, brain, and pancreas. Isoform Alpha is highly expressed in embryos from day 7-11 and in adul
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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