Recombinant Mouse LIM domain and actin-binding protein 1 (Lima1)

Code CSB-YP887660MO
MSDS
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Source Yeast
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Code CSB-EP887660MO
MSDS
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Source E.coli
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Code CSB-EP887660MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887660MO
MSDS
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Source Baculovirus
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Code CSB-MP887660MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lima1
Uniprot No.
Alternative Names
Lima1; D15Ertd366e; EplinLIM domain and actin-binding protein 1; Epithelial protein lost in neoplasm; mEPLIN
Species
Mus musculus (Mouse)
Expression Region
1-753
Target Protein Sequence
MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE DEE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake.
Gene References into Functions
  1. this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption. PMID: 29880681
  2. findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN. PMID: 25609703
  3. Eplin-alpha transcription is regulated by actin-MAL-SRF signalling. PMID: 20236507
  4. EPLIN may function in growth control by associating with and regulating the actin cytoskeleton PMID: 11950948
  5. ERK-mediated phosphorylation of EPLIN contributes to actin filament reorganization and enhanced cell motility. PMID: 17875928
  6. EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion PMID: 18093941

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell membrane. Cell projection, ruffle.
Tissue Specificity
Highly expressed in the small intestine, including the duodenum, jejunum, and ileum. Low expression in the liver and very low expressed in the heart, spleen, lung, brain, and pancreas. Isoform Alpha is highly expressed in embryos from day 7-11 and in adul
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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