lis-1 Antibody

Code CSB-PA873586XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lis-1 Polyclonal antibody
Uniprot No.
Target Names
lis-1
Alternative Names
lis-1 antibody; pnm-1 antibody; T03F6.5 antibody; Lissencephaly-1 homolog antibody; Pronuclear migration abnormal protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lis-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division. Part of a complex with nud-2, which is recruited to the nuclear envelope by unc-83, where, in turn, it recruits dynein to the nuclear surface and regulates nuclear migration in hypodermal precursor cells. Plays a role in GABAergic synaptic vesicle localization in the ventral nerve cord. Required for neuronal cell differentiation.
Gene References into Functions
  1. The convulsions mimicking epilepsy can be induced by a mutation in a C. elegans lis-1 allele (pnm-1), in combination with a chemical antagonist of gamma-aminobutyric acid (GABA) neurotransmitter signaling. PMID: 15254012
  2. Dynein and Lis1, albeit functioning in identical processes, are targeted partially independently of one another. PMID: 15331665
  3. lis-1 induces a spindle checkpoint-dependent mitotic arrest in the germline and an increase in germ-cell apoptosis. PMID: 17376425
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus envelope.
Protein Families
WD repeat LIS1/nudF family
Tissue Specificity
Expressed in all classes of neurons in the ventral cord. Expressed in the multinucleate spermathecal valves and adult seam cells.
Database Links

KEGG: cel:CELE_T03F6.5

STRING: 6239.T03F6.5

UniGene: Cel.19492

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