Recombinant Lissencephaly-1 homolog (lis-1)

Code CSB-YP873586CXY
MSDS
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Source Yeast
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Code CSB-EP873586CXY
MSDS
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Source E.coli
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Code CSB-EP873586CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873586CXY
MSDS
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Source Baculovirus
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Code CSB-MP873586CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lis-1
Uniprot No.
Alternative Names
lis-1; pnm-1; T03F6.5; Lissencephaly-1 homolog; Pronuclear migration abnormal protein 1
Species
Caenorhabditis elegans
Expression Region
1-404
Target Protein Sequence
MSLSERQKEE INRAIAEYMQ NNGYSESFSV FLKESSLSEN DIKPLGGILE KKWTTVLRLQ RKVNDLESKL QESQREINHG APTRDKRQAA DWIPRPPETQ KLTGHRLPIT RVIFHPLWTI MASCSEDATI KVWDYETGQL ERTLKGHTDA VNDIAIDAAG KQLVSCSSDL SIKLWDFGQT YDCLKSLKGH EHTVSSVTFL PTGDFVLSAS RDHTIKQWDI STGYCVYTFR GHNDWVRMIR ISNDGTLFAS ASLDQTVTVW SFATKSAKLV LRDHEHAVEC VEWAPDTAYT NVTGQQPEGN STHILFSGSR DRSIKAWNIN TGDVLFTLLA HENWVRGLAF HPKGKYLISV ADDKTLRVWE LSAQRCMKAI EAHEHFVSTV AFHQTSPFVI TGSVDMSCKV WECR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division. Part of a complex with nud-2, which is recruited to the nuclear envelope by unc-83, where, in turn, it recruits dynein to the nuclear surface and regulates nuclear migration in hypodermal precursor cells. Plays a role in GABAergic synaptic vesicle localization in the ventral nerve cord. Required for neuronal cell differentiation.
Gene References into Functions
  1. The convulsions mimicking epilepsy can be induced by a mutation in a C. elegans lis-1 allele (pnm-1), in combination with a chemical antagonist of gamma-aminobutyric acid (GABA) neurotransmitter signaling. PMID: 15254012
  2. Dynein and Lis1, albeit functioning in identical processes, are targeted partially independently of one another. PMID: 15331665
  3. lis-1 induces a spindle checkpoint-dependent mitotic arrest in the germline and an increase in germ-cell apoptosis. PMID: 17376425
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus envelope.
Protein Families
WD repeat LIS1/nudF family
Tissue Specificity
Expressed in all classes of neurons in the ventral cord. Expressed in the multinucleate spermathecal valves and adult seam cells.
Database Links

KEGG: cel:CELE_T03F6.5

STRING: 6239.T03F6.5

UniGene: Cel.19492

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