Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Lis-1 Polyclonal antibody

Uniprot No.

Q7KNS3

Target Names

Lis-1

Alternative Names

Lis-1 antibody; Lis1 antibody; CG8440 antibody; Lissencephaly-1 homolog antibody; DLis-1 antibody; Dlis1 antibody; Lissencephaly1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Lis-1 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division, mitotic spindle formation and the removal of mitotic checkpoint proteins from kinetochores at the metaphase to anaphase transition. Required for several aspects of neurogenesis including neuroblast proliferation, neuronal cell differentiation, dendritic growth, branching and maturation and axonal transport. Required for synchronized cell divisions in the germline, fusome integrity and oocyte differentiation. Acts together with BicD, Egl, dynein and microtubules to determine oocyte identity during oogenesis. Also required for nurse cell to oocyte transport during oocyte growth and for the positioning of the nucleus in the oocyte.

Gene References into Functions

lowering levels of Lissencephaly-1 (Lis1), a dynein motor co-factor, augments axonal mitochondrial transport in ageing wing neurons. PMID: 26598558
The data reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin. PMID: 23918939
A general role of Lis-1/NudE, Dynein and microtubules, in cell-on-cell migration, is reported. PMID: 22808215
present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during spermatogenesis PMID: 22764052
Lis1, which is widely expressed in adult mammalian tissues, could regulate adult tissue stem cells through modulating niche signaling and adhesion. PMID: 21041636
We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release. PMID: 16107559
Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces. PMID: 18485341

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Subcellular Location

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Note=Localizes to the plus end of microtubules. Also localizes to the mitotic spindle poles from late prophase through to telophase and to kinetochore microtubules in metaphase. Localization to kinetochore microtubules is reduced in anaphase and telophase. In oocytes, it concentrates in the cortex from stage 5 of oogenesis.

Protein Families

WD repeat LIS1/nudF family

Tissue Specificity

Expressed primarily in germline cells during oogenesis. Low levels are detected in the germarium in regions 1 and 2 and in both nurse cells and the oocyte in stage 2-4 egg chambers. Enriched in the oocyte during stages 5-7 and in nurse cells in stage 8-10

Database Links

KEGG: dme:Dmel_CG8440

STRING: 7227.FBpp0086375

UniGene: Dm.646

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Lis-1 Antibody

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