Recombinant Drosophila melanogaster Lissencephaly-1 homolog (Lis-1)

Code CSB-YP759065DLU
MSDS
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Source Yeast
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Code CSB-EP759065DLU
MSDS
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Source E.coli
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Code CSB-EP759065DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP759065DLU
MSDS
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Source Baculovirus
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Code CSB-MP759065DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lis-1
Uniprot No.
Alternative Names
Lis-1; Lis1; CG8440; Lissencephaly-1 homolog; DLis-1; Dlis1; Lissencephaly1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-411
Target Protein Sequence
MKMVLSQRQR EELNQAIADY LGSNGYADSL ETFRKEADLS TEVEKKFGGL LEKKWTSVIR LQKKVMELEA KLTEAEKEVI EGAPTKNKRT PGEWIPRPPE KFSLTGHRAS ITRVIFHPIF ALMVSASEDA TIRIWDFETG EYERSLKGHT DSVQDVAFDA QGKLLASCSA DLSIKLWDFQ QSYECIKTMH GHDHNVSSVA FVPAGDYVLS ASRDRTIKMW EVATGYCVKT YTGHREWVRM VRVHIEGSIF ATCSNDQTIR VWLTNSKDCK VELRDHEHTV ECIAWAPEAA ASAINEAAGA DNKKGHHQGP FLASGSRDKT IRIWDVSVGL CLLTLSGHDN WVRGLAFHPG GKYLVSASDD KTIRVWDLRN KRCMKTLYAH QHFCTSIDFH KAHPYVISGS VDQTVKVWEC R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division, mitotic spindle formation and the removal of mitotic checkpoint proteins from kinetochores at the metaphase to anaphase transition. Required for several aspects of neurogenesis including neuroblast proliferation, neuronal cell differentiation, dendritic growth, branching and maturation and axonal transport. Required for synchronized cell divisions in the germline, fusome integrity and oocyte differentiation. Acts together with BicD, Egl, dynein and microtubules to determine oocyte identity during oogenesis. Also required for nurse cell to oocyte transport during oocyte growth and for the positioning of the nucleus in the oocyte.
Gene References into Functions
  1. lowering levels of Lissencephaly-1 (Lis1), a dynein motor co-factor, augments axonal mitochondrial transport in ageing wing neurons. PMID: 26598558
  2. The data reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin. PMID: 23918939
  3. A general role of Lis-1/NudE, Dynein and microtubules, in cell-on-cell migration, is reported. PMID: 22808215
  4. present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during spermatogenesis PMID: 22764052
  5. Lis1, which is widely expressed in adult mammalian tissues, could regulate adult tissue stem cells through modulating niche signaling and adhesion. PMID: 21041636
  6. We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release. PMID: 16107559
  7. Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces. PMID: 18485341

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Note=Localizes to the plus end of microtubules. Also localizes to the mitotic spindle poles from late prophase through to telophase and to kinetochore microtubules in metaphase. Localization to kinetochore microtubules is reduced in anaphase and telophase. In oocytes, it concentrates in the cortex from stage 5 of oogenesis.
Protein Families
WD repeat LIS1/nudF family
Tissue Specificity
Expressed primarily in germline cells during oogenesis. Low levels are detected in the germarium in regions 1 and 2 and in both nurse cells and the oocyte in stage 2-4 egg chambers. Enriched in the oocyte during stages 5-7 and in nurse cells in stage 8-10
Database Links

KEGG: dme:Dmel_CG8440

STRING: 7227.FBpp0086375

UniGene: Dm.646

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