mael Antibody

Code CSB-PA893783XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) mael Polyclonal antibody
Uniprot No.
Target Names
mael
Alternative Names
mael antibody; CG11254Protein maelstrom antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) mael protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.
Gene References into Functions
  1. Study shows that the HMG protein Maelstrom is essential for Piwi-mediated silencing in Drosophila. PMID: 23159368
  2. Mael serves as a platform that nucleates other microtubule-organizing center components to form a functional MTOC in early oocyte development PMID: 22085963
  3. characterized a null allele of maelstrom (mael) and showed that PMID: 12538514
  4. Mael ensures proper differentiation in the germline stem cell lineage by repressing miR-7 PMID: 19758565
Subcellular Location
Cytoplasm. Nucleus. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Vas, aub and spn-E are required for nuage localization. Shuttles between the cytoplasm and the nucleus.
Protein Families
Maelstrom family
Tissue Specificity
In germaria and egg chambers, it is detected in the germline. In the germarium, it is in all regions, including region I where the germ cells are dividing. In early egg chambers, it is uniformly distributed throughout the nurse cells and oocyte but, by st
Database Links

KEGG: dme:Dmel_CG11254

STRING: 7227.FBpp0088574

UniGene: Dm.5827

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