Recombinant Drosophila melanogaster Protein maelstrom (mael)

Code CSB-YP893783DLU
MSDS
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Source Yeast
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Code CSB-EP893783DLU
MSDS
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Source E.coli
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Code CSB-EP893783DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893783DLU
MSDS
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Source Baculovirus
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Code CSB-MP893783DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mael
Uniprot No.
Alternative Names
mael; CG11254Protein maelstrom
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-459
Target Protein Sequence
MAPKKHSGFM MFVNEWRNRN AEGRRMTLAQ AVSHCGTIWE KMNTQQRGPY NSGGKDANVA QRAKRESSNG HGQVDKAQRE ATESLMDMKR TIERLVLNAK MSHDLENAKF VFVAFNYFTK ALTTDVYVPA EFAACEYSLK EGIRSIYSTM IDPGQIIFGQ GSDALLHSST THDLPLPPNA LGEKNMTKLY RNIVDYLSKC QGKGKTLVVF TPAENITMVK SCFRYLECDD DFRDGGEKIQ VFDIQYLLFI LKKEVMNVAD LNDEKINKFA TDAFFKKDFF EFTAGIACQY HEDNDRTKYC TQSMVTRWAY TFTDFMCGDL AITVQPGKHI PAQTKPNYLI ISSYASSLDH ESSFDSFYSL PGSGVKKESQ PEACSLSSSR LSVASSSYKP IDHTSFAANL NEVSEFPSLG MRNSSKHHGI AASAQREWNA RNLPTHSRLI RKVSDNDFSV NGADGKLKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.
Gene References into Functions
  1. Study shows that the HMG protein Maelstrom is essential for Piwi-mediated silencing in Drosophila. PMID: 23159368
  2. Mael serves as a platform that nucleates other microtubule-organizing center components to form a functional MTOC in early oocyte development PMID: 22085963
  3. characterized a null allele of maelstrom (mael) and showed that PMID: 12538514
  4. Mael ensures proper differentiation in the germline stem cell lineage by repressing miR-7 PMID: 19758565
Subcellular Location
Cytoplasm. Nucleus. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Vas, aub and spn-E are required for nuage localization. Shuttles between the cytoplasm and the nucleus.
Protein Families
Maelstrom family
Tissue Specificity
In germaria and egg chambers, it is detected in the germline. In the germarium, it is in all regions, including region I where the germ cells are dividing. In early egg chambers, it is uniformly distributed throughout the nurse cells and oocyte but, by st
Database Links

KEGG: dme:Dmel_CG11254

STRING: 7227.FBpp0088574

UniGene: Dm.5827

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