matP Antibody

Code CSB-PA359162XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) matP Polyclonal antibody
Uniprot No.
Target Names
matP
Alternative Names
matP antibody; ycbG antibody; b0956 antibody; JW0939Macrodomain Ter protein antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) matP protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for spatial organization of the terminus region of the chromosome (Ter macrodomain) during the cell cycle. Prevents early segregation of duplicated Ter macrodomains during cell division. Binds specifically to matS, which is a 13 bp signature motif repeated within the Ter macrodomain.
Gene References into Functions
  1. This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle. PMID: 27171414
  2. Data show that the replication terminus attaches to the divisome in a single segment along the perimeter of the cell in a MatP, ZapA and ZapB proteins-dependent manner. PMID: 26762981
  3. Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. PMID: 26818444
  4. studies revealing the molecular basis for Terminus-containing (Ter) chromosome condensation by the Ter-specific factor MatP PMID: 23084832
  5. demonstrated that MatP forms dimers that bind a single matS motif PMID: 22684059
  6. The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP (ycbG), DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. PMID: 18984159

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Subcellular Location
Cytoplasm. Note=Accumulates in the cell as a discrete focus that colocalizes with the Ter macrodomain.
Protein Families
MatP family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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