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Recombinant Escherichia coli Macrodomain Ter protein (matP)

Recombinant Escherichia coli Macrodomain Ter protein (matP)

Code	CSB-YP359162ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP359162ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP359162ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP359162ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP359162ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

matP

Uniprot No.

P0A8N0

Alternative Names

matP; ycbG; b0956; JW0939Macrodomain Ter protein

Species

Escherichia coli (strain K12)

Expression Region

1-150

Target Protein Sequence

MKYQQLENLE SGWKWKYLVK KHREGELITR YIEASAAQEA VDVLLSLENE PVLVNGWIDK HMNPELVNRM KQTIRARRKR HFNAEHQHTR KKSIDLEFIV WQRLAGLAQR RGKTLSETIV QLIEDAENKE KYANKMSSLK QDLQALLGKE

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for spatial organization of the terminus region of the chromosome (Ter macrodomain) during the cell cycle. Prevents early segregation of duplicated Ter macrodomains during cell division. Binds specifically to matS, which is a 13 bp signature motif repeated within the Ter macrodomain.

Gene References into Functions

This interaction is modulated by MatP, a protein involved in the organization of the Ter macrodomain. These results show that Topo IV, XerCD/dif and MatP are part of a network dedicated to the final step of chromosome management during the cell cycle. PMID: 27171414
Data show that the replication terminus attaches to the divisome in a single segment along the perimeter of the cell in a MatP, ZapA and ZapB proteins-dependent manner. PMID: 26762981
Impairing the TopoIV-MukBEF interaction delays sister ter segregation in cells lacking MatP. PMID: 26818444
studies revealing the molecular basis for Terminus-containing (Ter) chromosome condensation by the Ter-specific factor MatP PMID: 23084832
demonstrated that MatP forms dimers that bind a single matS motif PMID: 22684059
The effects of MatP inactivation reveal its role as main organizer of the Ter macrodomain: in the absence of MatP (ycbG), DNA is less compacted, the mobility of markers is increased, and segregation of Ter macrodomain occurs early in the cell cycle. PMID: 18984159