Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans mbk-2 Polyclonal antibody

Uniprot No.

Q9XTF3

Target Names

mbk-2

Alternative Names

mbk-2 antibody; F49E11.1Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 antibody; EC 2.7.12.1 antibody; Dual specificity Yak1-related kinase mbk-2 antibody; Minibrain Kinase 2 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans mbk-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Required for oocyte-to-zygote transition in which it phosphorylates oocyte proteins, including mei-1, oma-1, oma-2, mex-5, and mex-6, modifying their activity and/or stability following meiosis. Functions in both spindle positioning and in the posterior localization of cytoplasmic determinants, including pie-1, pos-1, and pgl-1, in early embryos. Involved in the asymmetric distribution of plk-1 at the 2-cell embryonic stage.

Gene References into Functions

Phosphorylation by MBK-2 facilitates the binding of OMA proteins to TAF-4 and simultaneously inactivates their function in repressing zif-1 translation during the specification of primordial germ cells. PMID: 20826530
MBK-2 functions as a temporal regulator of protein stability, and that coordinate activation of maternal protein degradation is one of the mechanisms that drives the transition from symmetric egg to patterned embryo. PMID: 12967564
mbk-2 is essential in the zygote to ensure the postmeiotic inactivation of MEI-1 possibly via direct phosphorylation regulation PMID: 14634695
MBK-2 down-regulates the katanin-related protein MEI-1 to control spindle positioning and, through yet unknown factors, to control the localization of cytoplasmic determinants. PMID: 14697358
MBK-2 phosphorylates OMA-1 in vitro, primarily at threonine239; phosphorylation at T239 is required both for OMA-1 function in the 1-cell embryo and its degradation after the first mitosis PMID: 16289132
MBK-2 directly phosphorylates MEI-1 and OMA-1 in vitro and that this activity is essential for their degradation in oocytes. PMID: 16338136

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Subcellular Location

Cytoplasm, cell cortex.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily

Tissue Specificity

In L1 larvae, expressed widely in the nervous system, including head neurons and the ventral nerve cord. In adult animals, continues to be expressed in the nervous system and is also expressed in body wall muscle.

Database Links

KEGG: cel:CELE_F49E11.1

STRING: 6239.F49E11.1b

UniGene: Cel.39641

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mbk-2 Antibody

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