Recombinant Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 (mbk-2), partial

Code CSB-YP895946CXY
MSDS
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Source Yeast
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Code CSB-EP895946CXY
MSDS
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Source E.coli
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Code CSB-EP895946CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895946CXY
MSDS
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Source Baculovirus
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Code CSB-MP895946CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mbk-2
Uniprot No.
Alternative Names
mbk-2; F49E11.1Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2; EC 2.7.12.1; Dual specificity Yak1-related kinase mbk-2; Minibrain Kinase 2
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for oocyte-to-zygote transition in which it phosphorylates oocyte proteins, including mei-1, oma-1, oma-2, mex-5, and mex-6, modifying their activity and/or stability following meiosis. Functions in both spindle positioning and in the posterior localization of cytoplasmic determinants, including pie-1, pos-1, and pgl-1, in early embryos. Involved in the asymmetric distribution of plk-1 at the 2-cell embryonic stage.
Gene References into Functions
  1. Phosphorylation by MBK-2 facilitates the binding of OMA proteins to TAF-4 and simultaneously inactivates their function in repressing zif-1 translation during the specification of primordial germ cells. PMID: 20826530
  2. MBK-2 functions as a temporal regulator of protein stability, and that coordinate activation of maternal protein degradation is one of the mechanisms that drives the transition from symmetric egg to patterned embryo. PMID: 12967564
  3. mbk-2 is essential in the zygote to ensure the postmeiotic inactivation of MEI-1 possibly via direct phosphorylation regulation PMID: 14634695
  4. MBK-2 down-regulates the katanin-related protein MEI-1 to control spindle positioning and, through yet unknown factors, to control the localization of cytoplasmic determinants. PMID: 14697358
  5. MBK-2 phosphorylates OMA-1 in vitro, primarily at threonine239; phosphorylation at T239 is required both for OMA-1 function in the 1-cell embryo and its degradation after the first mitosis PMID: 16289132
  6. MBK-2 directly phosphorylates MEI-1 and OMA-1 in vitro and that this activity is essential for their degradation in oocytes. PMID: 16338136

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Subcellular Location
Cytoplasm, cell cortex.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
Tissue Specificity
In L1 larvae, expressed widely in the nervous system, including head neurons and the ventral nerve cord. In adult animals, continues to be expressed in the nervous system and is also expressed in body wall muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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