VACWR117 Antibody

Code CSB-PA356237XA01VAI
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: VACWR117 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 34 kDa
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Product Details

Uniprot No.
Target Names
VACWR117
Alternative Names
mRNA-capping enzyme regulatory subunit (Virus termination factor small subunit) (VTF small subunit) (mRNA-capping enzyme 33 kDa subunit) (mRNA-capping enzyme D12 subunit) (mRNA-capping enzyme small subunit) VACWR117 D12L
Species Reactivity
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Immunogen
Recombinant Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR117 protein (49-128aa)
Immunogen Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The VACWR117 polyclonal antibody is produced by immunizing a rabbit with a recombinant Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR117 protein (49-128aa). The serum is collected from the rabbit to obtain polyclonal antibodies, which are then purified through protein G affinity chromatography. The efficacy of the VACWR117 antibody in recognizing (Vaccinia virus (strain WR)) VACWR117 protein is confirmed through ELISA and WB assays.

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Target Background

Function
Regulatory subunit of the mRNA cap enzyme which stabilizes the catalytic subunit and enhances its methyltransferase activity through an allosteric mechanism. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).; The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.
Gene References into Functions
  1. These results suggest that VTF may play a subtle role in early gene transcription elongation in addition to its known function in mRNA cap formation, early gene transcription termination and intermediate gene transcription initiation. PMID: 16460779
Subcellular Location
Virion. Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.
Protein Families
Chordopoxvirinae mRNA-capping enzyme regulatory subunit family
Database Links

KEGG: vg:3707515

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