Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RNMT Polyclonal antibody

Uniprot No.

O43148

Target Names

RNMT

Alternative Names

DKFZp686H1252 antibody; EC 2.1.1.56 antibody; hcm1p antibody; hCMT1 antibody; HCMT1a antibody; hCMT1c antibody; hMet antibody; hypothetical protein KIAA0396 antibody; KIAA0398 antibody; MCES_HUMAN antibody; MET antibody; mRNA (guanine 7)methyltransferase antibody; mRNA (guanine N(7)) methyltransferase antibody; mRNA (guanine-N(7)-)-methyltransferase antibody; mRNA cap guanine-N7 methyltransferase antibody; mRNA cap methyltransferase antibody; OTTHUMP00000162509 antibody; RG7MT1 antibody; RNA (guanine 7) methyltransferase antibody; RNA guanine 7 methyltransferase antibody; Rnmt antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) RNMT protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.

Gene References into Functions

Results identify a cytoplasmic pool of RNMT which is a component of the cytoplasmic capping complex, and demonstrate its function as the methyltransferase that catalyzes the final maturation step in cap homeostasis. PMID: 28981715
MYC promotes mRNA cap methylation and protein production of Wnt/beta-catenin signaling transcripts through recruitment of cyclin-dependent kinase 7 (CDK7) and consequently RNMT to gene promoters. PMID: 27899423
RNMT-RAM complex coordinates mRNA processing with ribosome production. PMID: 27934633
CDK1-Cyclin B1 activates RNMT, coordinating mRNA cap methylation with G1 phase transcription. PMID: 26942677
High RNMT expression is associated with liver cancer. PMID: 24763612
Data suggest the C-terminal nuclear localization domain (QYP) is critical for RAM (RNMT-activating mini protein) to enter the cell nucleus where RAM activates RNMT resulting in mRNA cap methylation. TNPO1/TNPO2 mediate RAM nuclear entry. PMID: 24200467
The RNMT N-terminal domain is required for transcript expression, translation and cell proliferation. PMID: 23863084
Nuclear localization of cap methyltransferase is mediated by alternative nuclear localization signal motifs PMID: 15767670

Hide All

Subcellular Location

Nucleus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0 methyltransferase family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 10075

OMIM: 603514

KEGG: hsa:8731

STRING: 9606.ENSP00000262173

UniGene: Hs.592347

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RNMT Antibody

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Target Background