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mcrB Antibody

Datasheet
Code CSB-PA323332XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) mcrB Polyclonal antibody
Uniprot No.
P15005
Target Names
mcrB
Alternative Names
mcrB antibody; rglB antibody; b4346 antibody; JW58715-methylcytosine-specific restriction enzyme B antibody; EC 3.1.21.- antibody; EcoKMcrBC antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
A synthesized peptide derived from Escherichia coli (strain K12) mcrB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, Dot blot (guaranteed specific to the immunizing peptide)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;
Quality Guarantee
1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

mcrB Proteins

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Target Background

Function
Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.
Gene References into Functions
  1. In the McrB-N structure in complex with methylated DNA, the 5mC base is flipped out from the DNA duplex and positioned within a binding pocket. PMID: 22570415
  2. Deletion of penicillin-binding protein 1b impairs biofilm formation and motility. PMID: 22349271
Database Links

KEGG: ecj:JW5871

STRING: 316407.85677086

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