Alternative Names
mcrB; rglB; b4346; JW58715-methylcytosine-specific restriction enzyme B; EC 3.1.21.-; EcoKMcrBC
Species
Escherichia coli (strain K12)
Target Protein Sequence
MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF AFLGEGQEAS
NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK TIAEYFQATS GVYPKKYGQS
YYACSQKVSQ GIDYTRFASM LDNIINDYKL IFNSGKSVIP PMSKTESYCL EDALNDLFIP
ETTIETILKR LTIKKNIILQ GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED
FIQGYRPNGV GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD
KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF SFIDIEPGFD
TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG KGFRIGHSYF CCGLEDGTSP
DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK WTNKLLGDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If
you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We
recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at
-20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Delivery time
may differ from different purchasing way or location, please kindly consult your local distributors
for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.
