At3g47520 Antibody

Code CSB-PA890397XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) At3g47520 Polyclonal antibody
Uniprot No.
Target Names
At3g47520
Alternative Names
At3g47520 antibody; F1P2.70 antibody; Malate dehydrogenase antibody; chloroplastic antibody; EC 1.1.1.37 antibody; Chloroplastic malate dehydrogenase antibody; Chloroplastic MDH antibody; cpNAD-MDH antibody; Plastidic NAD-dependent malate dehydrogenase antibody; pNAD-MDH antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana At3g47520 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability. Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well.
Gene References into Functions
  1. Purification and characterization of the plastid-localized NAD-dependent malate dehydrogenase from Arabidopsis thaliana PMID: 26095832
  2. The critical role of plastidial NAD-specific MDH isoform in Arabidopsis (Arabidopsis thaliana) plants, is reported. PMID: 24453164
  3. Arabidopsis thaliana mutants lacking the NADP-malate dehydrogenase have lost the reversible inactivation of catalase activity and the increase in H2O2 levels when exposed to high light. PMID: 24591715
  4. plastid-localized NAD-dependent malate dehydrogenase is crucial for energy homeostasis in developing Arabidopsis thaliana seeds PMID: 24198233
  5. mMDH has a role in maximizing photorespiratory rate. PMID: 20876337

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Subcellular Location
Plastid, chloroplast stroma.
Protein Families
LDH/MDH superfamily, MDH type 1 family
Tissue Specificity
Expressed in rosette leaves. Expressed in meristematic regions of roots and shoots, cotyledons, young leaves, trichomes, stamen, pollen, tapetum, gynoecium and ovules.
Database Links

KEGG: ath:AT3G47520

STRING: 3702.AT3G47520.1

UniGene: At.20474

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