Recombinant Arabidopsis thaliana Malate dehydrogenase, chloroplastic (At3g47520)

Code CSB-YP890397DOA
MSDS
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Source Yeast
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Code CSB-EP890397DOA
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Source E.coli
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Code CSB-EP890397DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890397DOA
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Source Baculovirus
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Code CSB-MP890397DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
At3g47520
Uniprot No.
Alternative Names
At3g47520; F1P2.70; Malate dehydrogenase; chloroplastic; EC 1.1.1.37; Chloroplastic malate dehydrogenase; Chloroplastic MDH; cpNAD-MDH; Plastidic NAD-dependent malate dehydrogenase; pNAD-MDH
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
81-403
Target Protein Sequence
ASYKVAVLGA AGGIGQPLSL LIKMSPLVST LHLYDIANVK GVAADLSHCN TPSQVRDFTG PSELADCLKD VNVVVIPAGV PRKPGMTRDD LFNINANIVK TLVEAVAENC PNAFIHIISN PVNSTVPIAA EVLKKKGVYD PKKLFGVTTL DVVRANTFVS QKKNLKLIDV DVPVIGGHAG ITILPLLSKT KPSVNFTDEE IQELTVRIQN AGTEVVDAKA GAGSATLSMA YAAARFVESS LRALDGDGDV YECSFVESTL TDLPFFASRV KIGKNGLEAV IESDLQGLTE YEQKALEALK VELKASIDKG VAFANKPAAA AAN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability. Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well.
Gene References into Functions
  1. Purification and characterization of the plastid-localized NAD-dependent malate dehydrogenase from Arabidopsis thaliana PMID: 26095832
  2. The critical role of plastidial NAD-specific MDH isoform in Arabidopsis (Arabidopsis thaliana) plants, is reported. PMID: 24453164
  3. Arabidopsis thaliana mutants lacking the NADP-malate dehydrogenase have lost the reversible inactivation of catalase activity and the increase in H2O2 levels when exposed to high light. PMID: 24591715
  4. plastid-localized NAD-dependent malate dehydrogenase is crucial for energy homeostasis in developing Arabidopsis thaliana seeds PMID: 24198233
  5. mMDH has a role in maximizing photorespiratory rate. PMID: 20876337

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Subcellular Location
Plastid, chloroplast stroma.
Protein Families
LDH/MDH superfamily, MDH type 1 family
Tissue Specificity
Expressed in rosette leaves. Expressed in meristematic regions of roots and shoots, cotyledons, young leaves, trichomes, stamen, pollen, tapetum, gynoecium and ovules.
Database Links

KEGG: ath:AT3G47520

STRING: 3702.AT3G47520.1

UniGene: At.20474

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