RECQL2 Antibody

Code CSB-PA880823XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RECQL2 Polyclonal antibody
Uniprot No.
Target Names
RECQL2
Alternative Names
RECQL2 antibody; MED34 antibody; MED34_1 antibody; RECQ2 antibody; RQL2 antibody; At1g31360 antibody; T19E23.16 antibody; ATP-dependent DNA helicase Q-like 2 antibody; EC 3.6.4.12 antibody; RecQ-like protein 2 antibody; AtRQ2 antibody; AtRecQ2 antibody; AtRecQl2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana RECQL2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
3'-5' DNA helicase that may play a role in the repair of DNA. Its DNA unwinding activity in vitro is dependent on magnesium, and ATP or dATP. Can use GTP/dGTP, CTP/dCTP or UTP/dUTP as nucleotide cofactors. Catalyzes Holliday junction branch migration and replication fork regression. Disrupts D-loop structures.
Gene References into Functions
  1. RecQ helicases AtRECQ2 and AtRECQ3 both show highly repetitive DNA unwinding. Although AtRECQ2 preferentially orients itself towards the junction to unwind it, AtRECQ3 moves in the opposite direction and processively rewinds, that is, helps closing, unzipped hairpins over hundreds of bases. Thus RecQ helicases can exhibit antagonistic activities on the molecular level. [RECQ2] PMID: 23771268
  2. AtRECQ2 is a (d)NTP-dependent 3'-->5' DNA helicase. AtRECQ2 is able to disrupt recombinogenic structures by disrupting various D-loop structures PMID: 18419780
Subcellular Location
Nucleus.
Protein Families
Helicase family, RecQ subfamily
Tissue Specificity
Expressed in shoots and flowers. Expressed in young leaves, inflorescences, roots, shoot apical meristem, young siliques, and mature green siliques.
Database Links

KEGG: ath:AT1G31360

STRING: 3702.AT1G31360.1

UniGene: At.40359

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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